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How-to · reconstitution

How to reconstitute BPC-157 10 mg

Put 2 mL of bacteriostatic water into the 10 mg BPC-157 vial. That makes 5.00 mg/mL. A 250 mcg dose is then 5.0 units on a U-100 syringe.

That volume is the one that lands the draw on a syringe line, so the reading can be checked by eye. More water makes a weaker mix and a bigger draw. Less water makes a stronger mix and a smaller one. The amount of BPC-157 in the glass never changes. Only the number of marks it takes does.

What matters first

Water to add
2 mL
Resulting strength
5.00 mg/mL
Example 250 mcg dose
5.0 U-100 units

What is BPC-157?

BPC-157 is a lab-made peptide, fifteen amino acids long, copied from one short stretch of a protein found in human stomach juice.

No completed controlled human trial has shown which dose works for BPC-157. The range people report using is not a study result, tested schedule or recommendation.

no human dose shown to work

Medically reviewed by Jennifer Montecillo, MD · non-practicing medical reviewerReviewed 2026-08-13open the primary sources

How much bacteriostatic water for a 10 mg BPC-157 vial?

◆ Unique to the 10 mg vialU-100 example draws
Water addedConcentrationPer U-100 unitExample: 250 mcg =
1 mL10.00 mg/mL100 mcg2.5 units
2 mL5.00 mg/mL50 mcg5.0 units
3 mL3.33 mg/mL33.33 mcg7.5 units

Straight arithmetic: concentration = 10 mg ÷ water, and 1 U-100 unit = 0.01 mL. The example column shows the math at 250 mcg; it is not a dose recommendation.

Which volume to use is a practical choice rather than a dose. Pick the one that puts your draw somewhere you can actually read it on the syringe. The flags in the last column mark where that fails. “Hard to measure” means the draw is too small to judge by eye. “Won’t fit” means it is bigger than one U-100 syringe holds.

BPC-157 10 mg vial: how much water to add. Each row is what that amount draws on a U-100 syringe. 1 mL makes 10.00 mg/mL, or 100 mcg a unit, and 250 mcg is 2.5 units. 2 mL makes 5.00 mg/mL, or 50 mcg a unit, and 250 mcg is 5 units. 3 mL makes 3.33 mg/mL, or 33.33 mcg a unit, and 250 mcg is 7.5 units. The barrel drawn here is filled to 5 units, the draw at 2 mL. The sum is 10 mg divided by the water. One U-100 unit is 0.01 mL.
The 10 mg vial at 2 mL on a U-100 barrel. 250 mcg fills to 5.0 units, at 5.00 mg/mL.

How many units is a documented dose from this vial?

The table above uses one worked example. These are the published quantities, converted into marks on a U-100 barrel at each water volume for a 10 mg vial. Where a quantity was never studied by injection, the row says so.

200–500 mcg — reported, not studied

200–500 mcg a day, by injection under the skin or by mouth. The review says this came from animal data, not a human dosing study.

Water addedConcentrationDraw, low to high
1 mL10.00 mg/mL2.0 to 5.0 units
2 mL5.00 mg/mL4.0 to 10.0 units
3 mL3.33 mg/mL6.0 to 15.0 units

indirect evidenceMateescu DM, Gavrilescu DM, Constantinescu FE, Oancea C, Ilie AC, Folescu R, Popa MD, Iurciuc S, Muresan CO, Enache A. BPC-157 as an Investigational Peptide Therapeutic: Biopharmaceutical Challenges, Formulation Strategies, and Translational Development Barriers. Pharmaceutics 2026;18(5):625 — states the quantity used off-label and that it was extrapolated informally from rodent data, with no human pharmacokinetic or safety anchor · retrieved 2026-08-13

Double the strength, and the readings stop landing on lines

With the same powder and water, doubling the dose doubles the draw. Two of the three water choices then leave the plunger between syringe lines.

3Read the full vial record — 3 notes

Only the middle volume reads cleanly

Of the three listed, one puts the draw on a whole number of units. The other two land on a half mark that no barrel prints. At a draw this small, half a mark is a large slice of what you are measuring.

This is the trade the bigger vial makes. Buying by the milligram looks like the thriftier move and it quietly buys a reading you cannot verify.

Least water is the worst combination available here

The strongest mix on this page produces the smallest draw on this page. Small draw, strong solution, no line to stop at: that is the arrangement that produces the errors, and it arrives on the largest vial rather than the smallest.

Adding water is the fix, and on a vial this size there is room to add it. Nothing about the compound changes; only whether the number can be trusted.

Forty draws with no expiry to bound them

This vial holds twice as many draws as its sibling and the page still gives no figure for how long the mixed liquid lasts. Whatever the true limit is, this is the vial most likely to run past it.

These facts do not show that the mixed vial remains stable. Nobody has published that test, and a larger vial may remain in use longer.

How do you mix it, step by step?

  1. Wipe both stoppers with an alcohol swab and let them dry.
  2. Draw up the water volume you picked from the table.
  3. Let the water run slowly down the inside wall of the BPC-157 vial, rather than jetting it onto the powder.
  4. Swirl gently until it dissolves. Shaking damages peptides.
  5. Store it as the dispensing label directs. What happens after mixing differs by product — some labels direct against refrigerating the mixed solution at all.

What if your water volume is not on the table?

The table shows the offered water volumes. The calculator does the same arithmetic for any volume and any syringe, and shows its working line by line.

Open the BPC-157 calculator →

Where to read next about BPC-157

The arithmetic on this page is only half the question. These are the pages that carry the other half.

How strong the evidence is for BPC-157

The strongest source for BPC-157 is no human dose shown to work.

Vial, water and dose for any peptide, with BPC-157 as one example

U-100, U-50 and U-40 barrels each read differently, including BPC-157

Every mixing and injection guide, including BPC-157

One page per vial size, because the math differs per vial, with BPC-157 as one example

Primary regulatory documents, including BPC-157

Every URL was fetched and live on its retrieval date, BPC-157 or otherwise.

1primary source, each with the day we read it — open the documents used on this page