Page last checked 2026-08-13

How-to · reconstitution

How to reconstitute BPC-157 5 mg

Put 1 mL of bacteriostatic water into the 5 mg BPC-157 vial. That makes 5.00 mg/mL. A 250 mcg dose is then 5.0 units on a U-100 syringe.

That volume is the one that lands the draw on a syringe line, so the reading can be checked by eye. More water makes a weaker mix and a bigger draw. Less water makes a stronger mix and a smaller one. The amount of BPC-157 in the glass never changes. Only the number of marks it takes does.

What matters first

Water to add: 1 mL
Resulting strength: 5.00 mg/mL
Example 250 mcg dose: 5.0 U-100 units

What is BPC-157?

BPC-157 is a lab-made peptide, fifteen amino acids long, copied from one short stretch of a protein found in human stomach juice.

No completed controlled human trial has shown which dose works for BPC-157. The range people report using is not a study result, tested schedule or recommendation.

no human dose shown to work

Medically reviewed by Jennifer Montecillo, MD · non-practicing medical reviewerReviewed 2026-08-13open the primary sources

How much bacteriostatic water for a 5 mg BPC-157 vial?

◆ Unique to the 5 mg vialU-100 example draws

Water added	Concentration	Per U-100 unit	Example: 250 mcg =
1 mL	5.00 mg/mL	50 mcg	5.0 units
2 mL	2.50 mg/mL	25 mcg	10.0 units
3 mL	1.67 mg/mL	16.67 mcg	15.0 units

Straight arithmetic: concentration = 5 mg ÷ water, and 1 U-100 unit = 0.01 mL. The example column shows the math at 250 mcg; it is not a dose recommendation.

Which volume to use is a practical choice rather than a dose. Pick the one that puts your draw somewhere you can actually read it on the syringe. The flags in the last column mark where that fails. “Hard to measure” means the draw is too small to judge by eye. “Won’t fit” means it is bigger than one U-100 syringe holds.

BPC-157 5 mg vial: how much water to add. Each row is what that amount draws on a U-100 syringe. 1 mL makes 5.00 mg/mL, or 50 mcg a unit, and 250 mcg is 5 units. 2 mL makes 2.50 mg/mL, or 25 mcg a unit, and 250 mcg is 10 units. 3 mL makes 1.67 mg/mL, or 16.67 mcg a unit, and 250 mcg is 15 units. The barrel drawn here is filled to 5 units, the draw at 1 mL. The sum is 5 mg divided by the water. One U-100 unit is 0.01 mL. — The 5 mg vial at 1 mL on a U-100 barrel. 250 mcg fills to 5.0 units, at 5.00 mg/mL.

How many units is a documented dose from this vial?

The table above uses one worked example. These are the published quantities, converted into marks on a U-100 barrel at each water volume for a 5 mg vial. Where a quantity was never studied by injection, the row says so.

200–500 mcg — reported, not studied

200–500 mcg a day, by injection under the skin or by mouth. The review says this came from animal data, not a human dosing study.

Water added	Concentration	Draw, low to high
1 mL	5.00 mg/mL	4.0 to 10.0 units
2 mL	2.50 mg/mL	8.0 to 20.0 units
3 mL	1.67 mg/mL	12.0 to 30.0 units

indirect evidenceMateescu DM, Gavrilescu DM, Constantinescu FE, Oancea C, Ilie AC, Folescu R, Popa MD, Iurciuc S, Muresan CO, Enache A. BPC-157 as an Investigational Peptide Therapeutic: Biopharmaceutical Challenges, Formulation Strategies, and Translational Development Barriers. Pharmaceutics 2026;18(5):625 — states the quantity used off-label and that it was extrapolated informally from rodent data, with no human pharmacokinetic or safety anchor · retrieved 2026-08-13

A readable draw, on a quantity no study produced

Every offered water volume puts the plunger on a printed syringe mark, making this vial easier to read. The amounts come from reported practice, not a dose established by a trial.

3Read the full vial record — 3 notes

Three volumes, three whole numbers

Not one of the listed volumes drops the plunger between graduations here. Each lands on a line, and the lines are far enough apart that a small hand tremor does not change what you have drawn.

That is worth naming because it is rare on a small research vial. The usual pattern at this scale is a draw of a few units with no line to stop at, and a reading that cannot be checked afterwards.

The vial is a long way ahead of the evidence

At the worked amount, one vial contains twenty draws. No study shows what twenty doses do, and the human evidence consists of three small uncontrolled reports involving about thirty people in total.

A vial that lasts is usually reassuring. Here it only means the arithmetic outruns the evidence sooner. The count is a fact about the glass, not about the compound.

Nothing tells you how long the mixed solution keeps

No cited source gives a storage window for a reconstituted vial, so no estimate is shown. This is an important unknown, not a number to guess.

With twenty draws in the vial, the mixed solution may sit for a long time before the powder runs out. How long it remains usable has not been measured.

How do you mix it, step by step?

Wipe both stoppers with an alcohol swab and let them dry.
Draw up the water volume you picked from the table.
Let the water run slowly down the inside wall of the BPC-157 vial, rather than jetting it onto the powder.
Swirl gently until it dissolves. Shaking damages peptides.
Store it as the dispensing label directs. What happens after mixing differs by product — some labels direct against refrigerating the mixed solution at all.

What if your water volume is not on the table?

The table shows the offered water volumes. The calculator does the same arithmetic for any volume and any syringe, and shows its working line by line.

Open the BPC-157 calculator →

Where to read next about BPC-157

The arithmetic on this page is only half the question. These are the pages that carry the other half.

BPC-157 — class, half-life and regulatory standing

No approved brand name is listed for BPC-157.

Every calculator on this site, BPC-157 and the rest

Every intermediate value is printed, for BPC-157 or any other vial.

The other guides written like the BPC-157 ones

Water options, resulting concentrations and an example draw for each, with BPC-157 as context

Where BPC-157 stands with the regulator, and when

Approval status, proceedings and the date attached to each, with BPC-157 as one example

1primary source, each with the day we read it — open the documents used on this page

Mateescu DM, Gavrilescu DM, Constantinescu FE, Oancea C, Ilie AC, Folescu R, Popa MD, Iurciuc S, Muresan CO, Enache A. BPC-157 as an Investigational Peptide Therapeutic: Biopharmaceutical Challenges, Formulation Strategies, and Translational Development Barriers. Pharmaceutics 2026;18(5):625 — states the quantity used off-label and that it was extrapolated informally from rodent data, with no human pharmacokinetic or safety anchorretrieved 2026-08-13indirect evidence