Page last checked 2026-08-13

How-to · reconstitution

How to reconstitute Semax 30 mg

Put 1 mL of bacteriostatic water into the 30 mg Semax vial. That makes 30.00 mg/mL. A 6 mg dose is then 20.0 units on a U-100 syringe.

That volume is the one that lands the draw on a syringe line, so the reading can be checked by eye. More water makes a weaker mix and a bigger draw. Less water makes a stronger mix and a smaller one. The amount of Semax in the glass never changes. Only the number of marks it takes does.

What matters first

Water to add: 1 mL
Resulting strength: 30.00 mg/mL
Example 6 mg dose: 20.0 U-100 units

What is Semax?

Semax is a peptide seven amino acids long, copied from a short stretch of a stress hormone. It carries none of that hormone’s own activity.

Medically reviewed by Jennifer Montecillo, MD · non-practicing medical reviewerReviewed 2026-08-13open the primary sources

How much bacteriostatic water for a 30 mg Semax vial?

◆ Unique to the 30 mg vialU-100 example draws

Water added	Concentration	Per U-100 unit	Example: 6 mg =
1 mL	30.00 mg/mL	300 mcg	20.0 units
2 mL	15.00 mg/mL	150 mcg	40.0 units

Straight arithmetic: concentration = 30 mg ÷ water, and 1 U-100 unit = 0.01 mL. The example column shows the math at 6 mg; it is not a dose recommendation.

Which volume to use is a practical choice rather than a dose. Pick the one that puts your draw somewhere you can actually read it on the syringe. The flags in the last column mark where that fails. “Hard to measure” means the draw is too small to judge by eye. “Won’t fit” means it is bigger than one U-100 syringe holds.

Semax 30 mg vial: how much water to add. Each row is what that amount draws on a U-100 syringe. 1 mL makes 30.00 mg/mL, or 300 mcg a unit, and 6 mg is 20 units. 2 mL makes 15.00 mg/mL, or 150 mcg a unit, and 6 mg is 40 units. The barrel drawn here is filled to 20 units, the draw at 1 mL. The sum is 30 mg divided by the water. One U-100 unit is 0.01 mL. — The 30 mg vial at 1 mL on a U-100 barrel. 6 mg fills to 20.0 units, at 30.00 mg/mL.

How many units is a documented dose from this vial?

The table above uses one worked example. These are the published quantities, converted into marks on a U-100 barrel at each water volume for a 30 mg vial. Where a quantity was never studied by injection, the row says so.

6 mg — studied in a trial

6 mg a day, in two courses of ten days separated by twenty days, into the nose. (110 patients at different stages after an ischemic stroke, mean age 58; the study also reported a brain-growth-factor marker in the blood rising alongside recovery scores).

Water added	Concentration	Draw
1 mL	30.00 mg/mL	20.0 units
2 mL	15.00 mg/mL	40.0 units

limited human dataGusev EI, Martynov MY, Kostenko EV, Petrova LV, Bobyreva SN. [The efficacy of semax in the tretament of patients at different stages of ischemic stroke]. Zh Nevrol Psikhiatr Im S S Korsakova. 2018;118(3 Vyp 2):61-68 — 110 patients, two ten-day courses of 6000 mcg a day separated by twenty days. Russian; read here through the English abstract indexed in PubMed · retrieved 2026-08-13

12 mg — studied in a trial

12 mg a day as a 1% solution, in two courses of ten days two weeks apart, into the nose. (27 patients; the report calls itself open-label. It states that Semax did not change the course of the disease, with a difference showing only on a quality-of-life total).

Water added	Concentration	Draw
1 mL	30.00 mg/mL	40.0 units
2 mL	15.00 mg/mL	80.0 units

limited human dataSerdiuk AV, Levitskii GN, Miasoedov NF, Skvortsova VI. [The study of chronic partial denervation and quality of life in patients with motor neuron disease treated with semax]. Zh Nevrol Psikhiatr Im S S Korsakova. 2007;107(4):29-39 — 27 patients given 12 mg a day intranasally as a 1% solution in two ten-day courses. The abstract calls it open-label and reports that Semax did not influence the course of the disease. Russian; read through the indexed English abstract · retrieved 2026-08-13

The larger Semax vial, where both volumes land on a line

This is the larger of the two vial strengths. Every water option fits in a U-100 syringe and lands exactly on a line. The vial contains several days at the worked amount.

3Read the full vial record — 3 notes

Neither volume is wrong to pick

Both listed volumes give a reading that sits on a syringe line, so the choice between them is about how much liquid goes under the skin rather than about whether the number can be read.

The tighter mix gives a small firm push. The looser one doubles the volume and halves the concentration, and both arrive at the same mass.

Several days of powder, and no window to spend it against

At the worked amount, this vial covers most of the published ten-day period. The smaller vial covers one day.

No published source says how long the mixed liquid lasts. The number of calculated draws is known; the usable storage window is not.

The quantity is real and it belongs to another product

The worked amount was published and given to a stated number of patients, making it better supported than a range based only on reported practice.

It was still given as drops into the nose, from a licensed bottle, in a study of stroke recovery. This vial is a research powder, and nothing published pairs the two.

How do you mix it, step by step?

Wipe both stoppers with an alcohol swab and let them dry.
Draw up the water volume you picked from the table.
Let the water run slowly down the inside wall of the Semax vial, rather than jetting it onto the powder.
Swirl gently until it dissolves. Shaking damages peptides.
Store it as the dispensing label directs. What happens after mixing differs by product — some labels direct against refrigerating the mixed solution at all.

What if your water volume is not on the table?

The table shows the offered water volumes. The calculator does the same arithmetic for any volume and any syringe, and shows its working line by line.

Open the Semax calculator →

Where to read next about Semax

The arithmetic on this page is only half the question. These are the pages that carry the other half.

What semax is, how it is used, and what has been studied

Semax is an acth(4-10) fragment analogue (nootropic, registered in russia).

Check the same draw on a vial that is not semax

Any vial at all, semax among them, with the arithmetic shown rather than hidden.

Where semax stands with the regulator, and when

Approval status, proceedings and the date attached to each, with semax as one example

Per-vial tables beyond the semax ones

Each guide uses the source for that peptide, not a general rule, including Semax

2primary sources, each with the day we read it — open the documents used on this page