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Reconstitution · GHK-Cu

GHK-Cu reconstitution calculator

Pick the vial, the water you added and the dose. Get the exact draw in units for the syringe in your hand.

Step 1 of 4

  1. How many mg are in your vial?
    Vial strengthamount printed on the vial
  2. How many mL of water did you add?
  3. What dose do you want to convert?
  4. Which syringe are you using?
Draw to
3.0units

0.03 mL · 16.67 mg/mL · 167 mcg per unit · 100 doses per vial

3.0 units on a U-100 insulin syringe · 1 mL · lines every 2 units, which is 0.03 milliliters. The nearest syringe line is 4 units.

This falls between two syringe lines. The nearest line on this U-100 syringe is 4 units. Open the syringe details below to see the difference.

50 mg ÷ 3 mL = 16.67 mg/mL
167 mcg/unit · 500 mcg = 0.030 mL = 3.0 units

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Show the math and syringe details

Can this syringe show the result clearly?

This draw falls between syringe lines. The exact volume is 3.00 units, but a U-100 syringe has a line every 2 units. Using the nearest line gives 4 units — a 33.3% difference, or 167 mcg.

Want an easier syringe number?

  • 2 mL of water instead25.00 mg/mL · 250 mcg/unit2.0 uon a syringe line
  • 2.5 mL of water instead20.00 mg/mL · 200 mcg/unit2.5 u20.0% off

Adding more water makes the mixture less concentrated, so the same dose uses more syringe units. It does not change how much peptide is in the vial.

This setup is in the address bar — every input is part of the link.

Educational math, not medical advice — dosing decisions belong to a licensed clinician. The tabs above also plan a schedule week by week and count how many doses one vial holds.

How to use the calculator

  1. STEP 1

    Enter the amount in the vial

    Use the total amount printed on the vial, in mg.

  2. STEP 2

    Enter the water you added

    Use the amount of water added to that vial, in mL.

  3. STEP 3

    Enter the dose you want to convert

    Choose mg or mcg, then match the result to your syringe.

GHK-Cu is a peptide three amino acids long holding a copper ion. It was pulled out of human blood plasma in 1973. It is sold for skin — wrinkles, firmness, scars — and for hair regrowth, and now increasingly as an injection.

Almost everything published is a cream on the surface of the skin. Where that work was blinded and measured objectively, the copper peptide did no better than its control. A 2026 review of injectable peptides says the dose, the frequency and the indications for injecting this one all remain unknown.

Examples using a 50 mg GHK-Cu vial

Each row starts with the same 50 mg vial and changes only the amount of water. More water makes the mixture less concentrated, so the same dose uses more syringe units.

◆ How the water amount changes the resultdose used: 500 mcg
For a 50 milligram vial: bacteriostatic water added, resulting concentration, micrograms per U-100 unit, and the draw for a 500 mcg dose.
Water addedConcentrationPer U-100 unitDraw for 500 mcgNearest syringe line
2 mL25.00 mg/mL250.0 mcg2.0 u · 0.020 mLexactly 2 u
3 mL16.67 mg/mL166.7 mcg3.0 u · 0.030 mL4 u · 33.3% off
5 mL10.00 mg/mL100.0 mcg5.0 u · 0.050 mL6 u · 20.0% off

The vial, water and dose shown here are the calculator’s starting example for GHK-Cu. Replace them with your own values. Every result in the table is calculated from those inputs.

Drawn from once a week at 500 mcg, a 50 mg vial yields 100 doses — about 100.0 weeks of supply. No cited source says how long the mixed vial keeps, so no storage window is shown.

A big vial, a strong solution, and very small draws

This vial holds many times the powder of a typical peptide vial, and it produces some of the smallest draws on the site. Both come from the same fact, and they pull against each other.

3Open the mixing details 3 notes

The most water on the page is the obvious choice

Even with the largest offered water volume, the solution stays highly concentrated. A typical amount falls within the first few syringe marks, where one mark changes the draw substantially.

The larger water option moves a small dose farther up the syringe, making the reading easier without changing the dose.

The blue is the compound

GHK-Cu is a short peptide holding a copper ion, and copper in solution is blue. A vial that turns blue on mixing has done what the chemistry says it should.

That is worth saying only so the color does not read as a fault. It is not a purity check and not a strength check in either direction. It is simply what this molecule looks like dissolved.

The mistake: turning a cream percentage into a vial dose

Nearly all the published human work on this molecule is a cream applied to skin, and its strengths are quoted as percentages of a preparation.

A percentage describes a mixture, not the amount a person received. It cannot be converted into a syringe draw without more information.

The one injected figure in circulation would empty vials at a time

People do ask what to inject, and a single number answers them across the internet. It is an author’s estimate, printed in a review. The listed employer of the person making it sells copper peptides for skin. Set beside the vial, the figure is not close to what this table works with.

4Read the supporting evidence 4 supporting notes

An estimate, and the review says as much in its own words

The sentence people quote begins with the authors estimating. They reached the figure by inference from injections given to animals. An estimate is a judgment about what might work. It is not a record of what anyone received.

Thousands of people are injected with it, and nobody prints how much

Two published studies between them cover many thousands of scalp injections. One ran monthly for months in a very large group of men. The other ran every three weeks in a group of a thousand patients.

Every injection with a number attached went into a compartment

The scalp work put it into skin. The animal work put it into the abdominal cavity of mice, into a rat knee joint, and into a chamber implanted under a rat’s skin.

Those scalp intervals are long, and this page has no clock

The two large studies dosed monthly, or every few weeks. Intervals that long change what a mixed vial is. It stops being a week’s mixing and becomes something standing in a fridge for months.

Units to dose at 16.67 mg/mL

Mix 50 mg into 3 mL and one U-100 unit carries 166.7 mcg. The table shows how much peptide is in each numbered syringe line. These are conversion examples, not suggested doses.

10Open the unit conversion table 10 rows
Numbered U-100 syringe lines converted to volume and mass at 16.67 milligrams per milliliter.
U-100 lineVolumeMass (mcg)Mass (mg)
10 u0.10 mL1,667 mcg1.667 mg
20 u0.20 mL3,333 mcg3.333 mg
30 u0.30 mL5,000 mcg5 mg
40 u0.40 mL6,667 mcg6.667 mg
50 u0.50 mL8,333 mcg8.333 mg
60 u0.60 mL10,000 mcg10 mg
70 u0.70 mL11,667 mcg11.667 mg
80 u0.80 mL13,333 mcg13.333 mg
90 u0.90 mL15,000 mcg15 mg
100 u1.00 mL16,667 mcg16.667 mg

How the math works

  1. Concentration — divide the amount in the vial by the water you added: mg ÷ mL = mg/mL. This tells you how many mg are in each mL.
  2. Volume — the dose divided by that concentration: dose ÷ (mg/mL) = mL.
  3. Units — on a U-100 syringe one unit is 0.01 mL, so mL × 100 = units. A U-40 unit is 0.025 mL, so the wrong barrel is a 2.5× error. That is why the calculator asks which one you have.
  4. Rounding gap — the exact draw against the nearest syringe line: |exact − nearest line| ÷ exact × 100 = % difference.

Medically reviewed by Jennifer Montecillo, MD · non-practicing medical reviewerReviewed 2026-08-13

Useful guides for this calculation

The math is only one part of the question. These guides cover the compound, published doses, side effects, mixing steps and supplies.