Page last checked 2026-09-07

How-to · reconstitution

How to reconstitute AOD-9604 5 mg

In this example, 2 mL of bacteriostatic water in a 5 mg AOD-9604 vial gives 2.50 mg/mL. An example 1 mg amount is then 40.0 units on a U-100 syringe.

This example lands on a syringe line. More water makes a weaker mix and a bigger draw. Less water makes a stronger mix and a smaller one. The amount of AOD-9604 stays the same. Use the diluent and volume specified for your product; the arithmetic alone does not choose them.

What matters first

Example water volume: 2 mL
Resulting strength: 2.50 mg/mL
Example 1 mg dose: 40.0 U-100 units

How much bacteriostatic water for a 5 mg AOD-9604 vial?

◆ Unique to the 5 mg vialU-100 example draws

Water added	Concentration	Per U-100 unit	Example: 1 mg =
2 mL	2.50 mg/mL	25 mcg	40.0 units
3 mL	1.67 mg/mL	16.67 mcg	60.0 units

Straight arithmetic: concentration = 5 mg ÷ liquid volume, and 1 U-100 unit = 0.01 mL. The example column shows the math at 1 mg; it is not a dose recommendation.

The table compares possible concentrations; it does not establish which diluent or volume suits your product. Follow its dispensing instructions.

“Hard to measure” means the example draw is below two U-100 units. “Won’t fit” means it exceeds this syringe’s capacity.

AOD-9604 5 mg vial: example mixing volumes. Each row is what that amount draws on a U-100 syringe. 2 mL makes 2.50 mg/mL, or 25 mcg a unit, and 1 mg is 40 units. 3 mL makes 1.67 mg/mL, or 16.67 mcg a unit, and 1 mg is 60 units. The barrel drawn here is filled to 40 units, the draw at 2 mL. The sum is 5 mg divided by the liquid volume. One U-100 unit is 0.01 mL. — The 5 mg vial at 2 mL on a U-100 barrel. 1 mg fills to 40.0 units, at 2.50 mg/mL.

Medically reviewed by Kenneth Montecillo, MD2026-09-07open the primary sources

What is AOD-9604?

AOD-9604 is the tail end of human growth hormone — residues 176 to 191, with one extra amino acid added on the front.

How many units is a documented dose from this vial?

The table above uses one worked example. These are the published quantities, converted into marks on a U-100 barrel at each listed liquid volume for a 5 mg vial. Where a quantity was never studied by injection, the row says so.

1–10 mg — reported, not studied

1–10 mg once daily over 12 weeks, swallowed. The trials did not inject it, the 1 mg group lost 2.6 kg against 0.8 kg on placebo, the 10 mg group lost less than the 1 mg group, and the primary trial reports were never published.

Water added	Concentration	Draw, low to high
2 mL	2.50 mg/mL	40.0 to 400.0 unitswon’t fit
3 mL	1.67 mg/mL	60.0 to 600.0 unitswon’t fit

limited human dataValentino MA, Lin JE, Waldman SA. Central and peripheral molecular targets for antiobesity pharmacotherapy. Clin Pharmacol Ther 2010;87(6):652-62 — reports the AOD-9604 12-week trial arms (1 mg/day and 10 mg/day) and the 536-subject 24-week trial that ended development · retrieved 2026-08-12

250–500 mcg — reported, not studied

250–500 mcg a day, split over two or three injections under the skin. The human trials swallowed this compound rather than injecting it, and at roughly ten times the quantity.

Water added	Concentration	Draw, low to high
2 mL	2.50 mg/mL	10.0 to 20.0 units
3 mL	1.67 mg/mL	15.0 to 30.0 units

indirect evidenceDominikowski A, Rękoś Z, Olejarz M, Szczepanek-Parulska E, Domin R, Ruchała M. The emerging landscape of performance-enhancing peptides modulating GH-IGF1 axis: bridging the gap between clinical evidence and patient self-administration. Front Endocrinol 2026 — tabulates self-reported online dosing patterns beside the clinical doses, stating the reported values must not be read as clinically validated, regulatory-approved or safe · retrieved 2026-08-12

Five worked doses from an amount reported in oral trials

One vial size, two water volumes, and a milligram-scale reading that sits well up the syringe at either. The reported oral trial amount is not a validated injection dose.

3Read the full vial record — 3 notes

Both volumes land on marks, high on the barrel

The worked amount is much larger than the figures on most peptide pages. The draw therefore covers more of the syringe and lands exactly on a line with either water option.

These inputs do not require rounding to a whole-unit mark. That does not address the separate question of whether a dose, route or preparation is supported by evidence.

The human trials were taken by mouth

The controlled studies behind this fragment had participants swallow a daily amount for twelve weeks. Those oral weight-loss results do not establish an injection regimen.

An oral study amount cannot be treated as an equivalent injection dose. The calculation converts a mass to a liquid volume; it does not measure absorption or bridge the difference between those routes.

Five draws, and more was not better

The vial divides into five worked doses, but no product-specific storage limit is assigned to the example.

In the trials the larger group lost less weight than the smaller one. The bigger studies did not separate the fragment from placebo at all. Reading the top of the range as a target reads the evidence backwards.

Those trials were never published as primary papers either. The quantities on this page come from a peer-reviewed review of a company announcement, which is why the page calls them reported rather than studied.

How do you mix it, step by step?

Wipe both stoppers with an alcohol swab and let them dry.
Check the product’s mixing instructions for the correct diluent and volume before drawing it up.
Let the water run slowly down the inside wall of the AOD-9604 vial, rather than jetting it onto the powder.
Follow the product’s instructions for mixing and checking the solution before use. Do not assume every powder uses the same method.
Store it as the dispensing label directs. What happens after mixing differs by product — some labels direct against refrigerating the mixed solution at all.

What if your water volume is not on the table?

The calculator lets you enter your product’s vial strength and mixing volume, then shows the arithmetic for your syringe.

Open the AOD-9604 calculator →

Questions readers ask

How much water to add to reconstitute peptides?

Use the amount printed on the pharmacy label. In the worked example, 1 mg draws 40 units with 2 mL of water or 60 units with 3 mL. Water changes the concentration, not the dose.

Both land on a printed line, and both sit high on the barrel. That comfort comes from a milligram-scale amount rather than from the compound.

How to reconstitute AOD-9604 pdf?

There is no PDF, and this site does not publish one. What it has instead is a print card built into the calculator.

The card prints the mix, the draw, a barrel drawn to scale with the draw marked, and a blank log grid. It leaves the reconstitution date as an empty line, because inventing a date would invent a discard date with it.

What to reconstitute aOD with?

Bacteriostatic water — every volume on this page is a volume of it, and the two listed are 2 mL and 3 mL.

No published source names another liquid for this fragment. The human trials used an oral form; nothing published describes injecting it.

Do you refrigerate AOD-9604?

Nobody has published a storage instruction this site can point to for AOD-9604. The storage records here cover four branded pens and nothing else.

This page also carries no beyond-use figure, so there is no sourced window for how long a mixed vial keeps. Borrowing a fridge rule from a different product would mean inventing one.

Where to read next about AOD-9604

The arithmetic on this page is only half the question. These are the pages that carry the other half.

AOD-9604 — class, half-life and regulatory standing

No approved brand name is listed for AOD-9604.

Check the same draw on a vial that is not AOD-9604

Any vial at all, AOD-9604 among them, with the arithmetic shown rather than hidden.

Find AOD-9604 in the mixing and injection guides

Browse the available mixing and injection guides for more context on AOD-9604.

The proceedings behind the AOD-9604 note on this page

Newest entry first, with AOD-9604 among them.

2primary sources, each with the day we read it — open the documents used on this page