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How-to · reconstitution

How to reconstitute DSIP 10 mg

Put 2 mL of bacteriostatic water into the 10 mg DSIP vial. That makes 5.00 mg/mL. A 250 mcg dose is then 5.0 units on a U-100 syringe.

That volume is the one that lands the draw on a syringe line, so the reading can be checked by eye. More water makes a weaker mix and a bigger draw. Less water makes a stronger mix and a smaller one. The amount of DSIP in the glass never changes. Only the number of marks it takes does.

What matters first

Water to add
2 mL
Resulting strength
5.00 mg/mL
Example 250 mcg dose
5.0 U-100 units

What is DSIP?

DSIP stands for delta sleep-inducing peptide. It is nine amino acids long, and it was recovered in 1977 from the blood of rabbits put into deep sleep electrically.

Medically reviewed by Jennifer Montecillo, MD · non-practicing medical reviewerReviewed 2026-08-12open the primary sources

How much bacteriostatic water for a 10 mg DSIP vial?

◆ Unique to the 10 mg vialU-100 example draws
Water addedConcentrationPer U-100 unitExample: 250 mcg =
2 mL5.00 mg/mL50 mcg5.0 units
3 mL3.33 mg/mL33.33 mcg7.5 units

Straight arithmetic: concentration = 10 mg ÷ water, and 1 U-100 unit = 0.01 mL. The example column shows the math at 250 mcg; it is not a dose recommendation.

Which volume to use is a practical choice rather than a dose. Pick the one that puts your draw somewhere you can actually read it on the syringe. The flags in the last column mark where that fails. “Hard to measure” means the draw is too small to judge by eye. “Won’t fit” means it is bigger than one U-100 syringe holds.

DSIP 10 mg vial: how much water to add. Each row is what that amount draws on a U-100 syringe. 2 mL makes 5.00 mg/mL, or 50 mcg a unit, and 250 mcg is 5 units. 3 mL makes 3.33 mg/mL, or 33.33 mcg a unit, and 250 mcg is 7.5 units. The barrel drawn here is filled to 5 units, the draw at 2 mL. The sum is 10 mg divided by the water. One U-100 unit is 0.01 mL.
The 10 mg vial at 2 mL on a U-100 barrel. 250 mcg fills to 5.0 units, at 5.00 mg/mL.

Forty draws, and the readings fall into single figures

The larger DSIP vial doubles the doses and halves the draw. At the smaller water volume that puts one dose at five units, where a single mark is a fifth of it.

3Read the full vial record 3 notes

More powder, less legible answer

Doubling the vial at the same water doubles the strength, so the same amount takes half the liquid and half the marks. The count of doses goes up and the quality of the reading goes down.

On this page that trade is unusually stark. The draws were already small on the smaller vial.

The larger volume does not land on a mark

Of the two listed, one gives a whole number of units. The other leaves the plunger on a half-mark the barrel never prints.

Neither option is easy to measure: one is very small, while the other falls between syringe lines. Both exact results are shown without recommending one.

Forty draws against no window at all

The vial divides into forty worked amounts, but no published source says how long the mixed solution keeps.

The human studies behind this peptide date from the 1980s. They put it into a vein at amounts scaled to body weight, and none of them reached an approval. The count below is arithmetic on a vial, not a course anyone has run.

How do you mix it, step by step?

  1. Wipe both stoppers with an alcohol swab and let them dry.
  2. Draw up the water volume you picked from the table.
  3. Let the water run slowly down the inside wall of the DSIP vial, rather than jetting it onto the powder.
  4. Swirl gently until it dissolves. Shaking damages peptides.
  5. Store it as the dispensing label directs. What happens after mixing differs by product — some labels direct against refrigerating the mixed solution at all.

What if your water volume is not on the table?

The table shows the offered water volumes. The calculator does the same arithmetic for any volume and any syringe, and shows its working line by line.

Open the DSIP calculator →

Where to read next about DSIP

The arithmetic on this page is only half the question. These are the pages that carry the other half.

2primary sources, each with the day we read it — open the documents used on this page