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How-to · reconstitution

How to reconstitute DSIP 5 mg

In this example, 2 mL of bacteriostatic water in a 5 mg DSIP vial gives 2.50 mg/mL. An example 250 mcg amount is then 10.0 units on a U-100 syringe.

This example lands on a syringe line. More water makes a weaker mix and a bigger draw. Less water makes a stronger mix and a smaller one. The amount of DSIP stays the same. Use the diluent and volume specified for your product; the arithmetic alone does not choose them.

What matters first

Example water volume
2 mL
Resulting strength
2.50 mg/mL
Example 250 mcg dose
10.0 U-100 units

How much bacteriostatic water for a 5 mg DSIP vial?

◆ Unique to the 5 mg vialU-100 example draws
Water addedConcentrationPer U-100 unitExample: 250 mcg =
2 mL2.50 mg/mL25 mcg10.0 units
3 mL1.67 mg/mL16.67 mcg15.0 units

Straight arithmetic: concentration = 5 mg ÷ liquid volume, and 1 U-100 unit = 0.01 mL. The example column shows the math at 250 mcg; it is not a dose recommendation.

The table compares possible concentrations; it does not establish which diluent or volume suits your product. Follow its dispensing instructions.

“Hard to measure” means the example draw is below two U-100 units. “Won’t fit” means it exceeds this syringe’s capacity.

DSIP 5 mg vial: example mixing volumes. Each row is what that amount draws on a U-100 syringe. 2 mL makes 2.50 mg/mL, or 25 mcg a unit, and 250 mcg is 10 units. 3 mL makes 1.67 mg/mL, or 16.67 mcg a unit, and 250 mcg is 15 units. The barrel drawn here is filled to 10 units, the draw at 2 mL. The sum is 5 mg divided by the liquid volume. One U-100 unit is 0.01 mL.
The 5 mg vial at 2 mL on a U-100 barrel. 250 mcg fills to 10.0 units, at 2.50 mg/mL.

Medically reviewed by Kenneth Montecillo, MD2026-09-07open the primary sources

What is DSIP?

DSIP stands for delta sleep-inducing peptide. It is nine amino acids long, and it was recovered in 1977 from the blood of rabbits put into deep sleep electrically.

The smaller DSIP vial is the one that reads

The vial contains twenty worked doses. Both water options land exactly on syringe lines and sit above the crowded bottom of the syringe.

3Read the full vial record — 3 notes

The vial size moves the reading further than the water does

Between this page’s two water volumes the draw changes by half again. Between its two vial sizes it doubles.

Both inputs matter when matching a unit reading to a dose. A change in vial strength cannot be ignored just because the water amount is familiar; check the resulting concentration as well as the final number.

Twenty draws, both of them on marks

Each listed volume gives a whole number of units here. The liquid volume changes the draw for each dose, but does not change how many doses the entered drug mass contains or how long the product remains usable.

No storage limit is assigned to this worked preparation. Twenty is a dose count, not twenty weeks of use.

The strength on the glass is a seller’s claim

The calculation uses the drug content entered from the vial label. It cannot independently confirm the identity, amount, purity or sterility of the material in a research product.

Every figure on this page inherits that assertion. The arithmetic cannot be more reliable than the quantity it starts from.

A precise result is therefore not a quality check on the vial. It shows the relationship between the stated inputs, and would change if the actual contents differed from those inputs.

How do you mix it, step by step?

  1. Wipe both stoppers with an alcohol swab and let them dry.
  2. Check the product’s mixing instructions for the correct diluent and volume before drawing it up.
  3. Let the water run slowly down the inside wall of the DSIP vial, rather than jetting it onto the powder.
  4. Follow the product’s instructions for mixing and checking the solution before use. Do not assume every powder uses the same method.
  5. Store it as the dispensing label directs. What happens after mixing differs by product — some labels direct against refrigerating the mixed solution at all.

What if your water volume is not on the table?

The calculator lets you enter your product’s vial strength and mixing volume, then shows the arithmetic for your syringe.

Open the DSIP calculator →

Where to read next about DSIP

The arithmetic on this page is only half the question. These are the pages that carry the other half.

2primary sources, each with the day we read it — open the documents used on this page