Page last checked 2026-08-13

How-to · reconstitution

How to reconstitute Epitalon 10 mg

Put 1 mL of bacteriostatic water into the 10 mg Epitalon vial. That makes 10.00 mg/mL. A 0.5 mg dose is then 5.0 units on a U-100 syringe.

That volume is the one that lands the draw on a syringe line, so the reading can be checked by eye. More water makes a weaker mix and a bigger draw. Less water makes a stronger mix and a smaller one. The amount of Epitalon in the glass never changes. Only the number of marks it takes does.

What matters first

Water to add: 1 mL
Resulting strength: 10.00 mg/mL
Example 0.5 mg dose: 5.0 U-100 units

What is Epitalon?

Epitalon is a peptide four amino acids long, made in Leningrad in the 1980s. It copies the amino-acid makeup of an extract of cow pineal gland.

Medically reviewed by Jennifer Montecillo, MD · non-practicing medical reviewerReviewed 2026-08-13open the primary sources

How much bacteriostatic water for a 10 mg Epitalon vial?

◆ Unique to the 10 mg vialU-100 example draws

Water added	Concentration	Per U-100 unit	Example: 0.5 mg =
1 mL	10.00 mg/mL	100 mcg	5.0 units
2 mL	5.00 mg/mL	50 mcg	10.0 units
3 mL	3.33 mg/mL	33.33 mcg	15.0 units

Straight arithmetic: concentration = 10 mg ÷ water, and 1 U-100 unit = 0.01 mL. The example column shows the math at 0.5 mg; it is not a dose recommendation.

Which volume to use is a practical choice rather than a dose. Pick the one that puts your draw somewhere you can actually read it on the syringe. The flags in the last column mark where that fails. “Hard to measure” means the draw is too small to judge by eye. “Won’t fit” means it is bigger than one U-100 syringe holds.

Epitalon 10 mg vial: how much water to add. Each row is what that amount draws on a U-100 syringe. 1 mL makes 10.00 mg/mL, or 100 mcg a unit, and 0.5 mg is 5 units. 2 mL makes 5.00 mg/mL, or 50 mcg a unit, and 0.5 mg is 10 units. 3 mL makes 3.33 mg/mL, or 33.33 mcg a unit, and 0.5 mg is 15 units. The barrel drawn here is filled to 5 units, the draw at 1 mL. The sum is 10 mg divided by the water. One U-100 unit is 0.01 mL. — The 10 mg vial at 1 mL on a U-100 barrel. 0.5 mg fills to 5.0 units, at 10.00 mg/mL.

How many units is a documented dose from this vial?

The table above uses one worked example. These are the published quantities, converted into marks on a U-100 barrel at each water volume for a 10 mg vial. Where a quantity was never studied by injection, the row says so.

0.5 mg — studied in a trial

0.5 mg once a day for twenty days, under the tongue. (75 women, with a placebo group and an untreated group; the endpoints were a melatonin marker in urine and three clock genes in blood cells).

Water added	Concentration	Draw
1 mL	10.00 mg/mL	5.0 units
2 mL	5.00 mg/mL	10.0 units
3 mL	3.33 mg/mL	15.0 units

limited human dataAraj SK, Brzezik J, Mądra-Gackowska K, Szeleszczuk Ł. Overview of Epitalon—Highly Bioactive Pineal Tetrapeptide with Promising Properties. Int J Mol Sci. 2025;26(6):2691 — an independent Medical University of Warsaw review whose Clinical Trials section reports both human studies of the tetrapeptide with their doses, enrollments and comparison groups · retrieved 2026-08-13

5 mcg — studied in a trial

5 mcg per eye on ten days in a row, behind the eye. (162 patients aged 18 to 72 with retinitis pigmentosa; the comparison group got the usual treatments of 2002, and no treated patient reported a side effect).

Water added	Concentration	Draw
1 mL	10.00 mg/mL	0.1 unitshard to measure
2 mL	5.00 mg/mL	0.1 unitshard to measure
3 mL	3.33 mg/mL	0.1 unitshard to measure

One vial, and the studied amount arrived by a different route

The quantity this table converts is the only whole-body amount published for a person taking this peptide, and it was held under the tongue rather than injected. The single vial size makes the water column the only choice this page offers.

3Read the full vial record — 3 notes

A conversion is not a bridge between routes

The sum on this page turns a mass into a volume, correctly. It cannot tell you that the same mass under the skin does what it did under the tongue, because how much of a peptide reaches the blood depends on the way it goes in.

The other human study injected behind the eye, which is further still from a syringe in the abdomen. So this page converts a real number without any published measurement of the route it converts it for.

One vial size means the only decision here is water

Most compounds on this site offer a ladder of strengths, and choosing among them is half the arithmetic. This one has a single listed size, so everything on the page turns on how much liquid the powder goes into.

All three volumes land the plunger on a printed line, so none of them is wrong to pick. The choice is between a small firm push and a larger, slower one.

Twenty draws against a twenty-day course

The published course ran once daily for twenty days, and the worked vial happens to contain twenty draws. That numerical match is a coincidence, not proof that the vial was designed for the course.

It is also the wrong course. That twenty-day run was taken by mouth. Nothing published pairs this vial with a length of time, and the page gives no figure for how long the mixed liquid keeps.

How do you mix it, step by step?

Wipe both stoppers with an alcohol swab and let them dry.
Draw up the water volume you picked from the table.
Let the water run slowly down the inside wall of the Epitalon vial, rather than jetting it onto the powder.
Swirl gently until it dissolves. Shaking damages peptides.
Store it as the dispensing label directs. What happens after mixing differs by product — some labels direct against refrigerating the mixed solution at all.

What if your water volume is not on the table?

The table shows the offered water volumes. The calculator does the same arithmetic for any volume and any syringe, and shows its working line by line.

Open the Epitalon calculator →

Where to read next about Epitalon

The arithmetic on this page is only half the question. These are the pages that carry the other half.

How strong the evidence is for epitalon

The strongest source for epitalon is a foreign approval or thin human data.

Take the epitalon arithmetic to any other vial

Concentration, units per draw and draws per vial, including Epitalon

The other guides written like the epitalon ones

Water options, resulting concentrations and an example draw for each, with epitalon as context

The dated regulatory page for epitalon and the rest

Every entry cites its source. It comes from the Federal Register or fda.gov, with epitalon as context

1primary source, each with the day we read it — open the documents used on this page

Araj SK, Brzezik J, Mądra-Gackowska K, Szeleszczuk Ł. Overview of Epitalon—Highly Bioactive Pineal Tetrapeptide with Promising Properties. Int J Mol Sci. 2025;26(6):2691 — an independent Medical University of Warsaw review whose Clinical Trials section reports both human studies of the tetrapeptide with their doses, enrollments and comparison groupsretrieved 2026-08-13limited human data