Page last checked 2026-09-07

How-to · reconstitution

How to reconstitute Epitalon 10 mg

In this example, 1 mL of bacteriostatic water in a 10 mg Epitalon vial gives 10.00 mg/mL. An example 0.5 mg amount is then 5.0 units on a U-100 syringe.

This example lands on a syringe line. More water makes a weaker mix and a bigger draw. Less water makes a stronger mix and a smaller one. The amount of Epitalon stays the same. Use the diluent and volume specified for your product; the arithmetic alone does not choose them.

What matters first

Example water volume: 1 mL
Resulting strength: 10.00 mg/mL
Example 0.5 mg dose: 5.0 U-100 units

How much bacteriostatic water for a 10 mg Epitalon vial?

◆ Unique to the 10 mg vialU-100 example draws

Water added	Concentration	Per U-100 unit	Example: 0.5 mg =
1 mL	10.00 mg/mL	100 mcg	5.0 units
2 mL	5.00 mg/mL	50 mcg	10.0 units
3 mL	3.33 mg/mL	33.33 mcg	15.0 units

Straight arithmetic: concentration = 10 mg ÷ liquid volume, and 1 U-100 unit = 0.01 mL. The example column shows the math at 0.5 mg; it is not a dose recommendation.

The table compares possible concentrations; it does not establish which diluent or volume suits your product. Follow its dispensing instructions.

“Hard to measure” means the example draw is below two U-100 units. “Won’t fit” means it exceeds this syringe’s capacity.

Epitalon 10 mg vial: example mixing volumes. Each row is what that amount draws on a U-100 syringe. 1 mL makes 10.00 mg/mL, or 100 mcg a unit, and 0.5 mg is 5 units. 2 mL makes 5.00 mg/mL, or 50 mcg a unit, and 0.5 mg is 10 units. 3 mL makes 3.33 mg/mL, or 33.33 mcg a unit, and 0.5 mg is 15 units. The barrel drawn here is filled to 5 units, the draw at 1 mL. The sum is 10 mg divided by the liquid volume. One U-100 unit is 0.01 mL. — The 10 mg vial at 1 mL on a U-100 barrel. 0.5 mg fills to 5.0 units, at 10.00 mg/mL.

Medically reviewed by Kenneth Montecillo, MD2026-09-07open the primary sources

What is Epitalon?

Epitalon is a peptide four amino acids long, made in Leningrad in the 1980s. It copies the amino-acid makeup of an extract of cow pineal gland.

How many units is a documented dose from this vial?

The table above uses one worked example. These are the published quantities, converted into marks on a U-100 barrel at each listed liquid volume for a 10 mg vial. Where a quantity was never studied by injection, the row says so.

0.5 mg — studied in a trial

0.5 mg once a day for twenty days, under the tongue. (75 women, with a placebo group and an untreated group; the endpoints were a melatonin marker in urine and three clock genes in blood cells).

Water added	Concentration	Draw
1 mL	10.00 mg/mL	5.0 units
2 mL	5.00 mg/mL	10.0 units
3 mL	3.33 mg/mL	15.0 units

limited human dataAraj SK, Brzezik J, Mądra-Gackowska K, Szeleszczuk Ł. Overview of Epitalon—Highly Bioactive Pineal Tetrapeptide with Promising Properties. Int J Mol Sci. 2025;26(6):2691 — an independent Medical University of Warsaw review whose Clinical Trials section reports both human studies of the tetrapeptide with their doses, enrollments and comparison groups · retrieved 2026-08-13

5 mcg — studied in a trial

5 mcg per eye on ten days in a row, behind the eye. (162 patients aged 18 to 72 with retinitis pigmentosa; the comparison group got the usual treatments of 2002, and no treated patient reported a side effect).

Water added	Concentration	Draw
1 mL	10.00 mg/mL	0.1 unitshard to measure
2 mL	5.00 mg/mL	0.1 unitshard to measure
3 mL	3.33 mg/mL	0.1 unitshard to measure

One vial, and the studied amount arrived by a different route

The worked amount comes from a cited study using a preparation under the tongue. Converting that amount into a syringe volume does not establish an equivalent injection dose or instructions for preparing the single vial example.

3Read the full vial record — 3 notes

A conversion is not a bridge between routes

The sum on this page turns a mass into a volume, correctly. It cannot tell you that the same mass under the skin does what it did under the tongue, because how much of a peptide reaches the blood depends on the way it goes in.

The other human study injected behind the eye, which is further still from a syringe in the abdomen. So this page converts a real number without any published measurement of the route it converts it for.

Three volume examples at one vial strength

Most compounds on this site offer a ladder of strengths, and choosing among them is half the arithmetic. This one has a single listed size, so everything on the page turns on how much liquid the powder goes into.

All three calculated readings land on whole-unit lines. That describes the arithmetic only; it does not show that any of the displayed dilutions is suitable or predict how an injection would feel.

Twenty draws against a twenty-day course

The published course ran once daily for twenty days, and the worked vial happens to contain twenty draws. That numerical match is a coincidence, not proof that the vial was designed for the course.

That course used a different route and preparation. Its duration does not validate storage or repeated injections from this vial example. A numerical match between a portion count and a course length does not make the products interchangeable.

How do you mix it, step by step?

Wipe both stoppers with an alcohol swab and let them dry.
Check the product’s mixing instructions for the correct diluent and volume before drawing it up.
Let the water run slowly down the inside wall of the Epitalon vial, rather than jetting it onto the powder.
Follow the product’s instructions for mixing and checking the solution before use. Do not assume every powder uses the same method.
Store it as the dispensing label directs. What happens after mixing differs by product — some labels direct against refrigerating the mixed solution at all.

What if your water volume is not on the table?

The calculator lets you enter your product’s vial strength and mixing volume, then shows the arithmetic for your syringe.

Open the Epitalon calculator →

Where to read next about Epitalon

The arithmetic on this page is only half the question. These are the pages that carry the other half.

How strong the evidence is for epitalon

The strongest source for epitalon is a foreign approval or thin human data.

Take the epitalon arithmetic to any other vial

Concentration, units per draw and draws per vial, including Epitalon

Browse epitalon guides by vial strength

Explore water volumes, concentrations and example draws alongside epitalon.

The dated regulatory page for epitalon and the rest

Every entry cites its source. It comes from the Federal Register or fda.gov, with epitalon as context

1primary source, each with the day we read it — open the documents used on this page

Araj SK, Brzezik J, Mądra-Gackowska K, Szeleszczuk Ł. Overview of Epitalon—Highly Bioactive Pineal Tetrapeptide with Promising Properties. Int J Mol Sci. 2025;26(6):2691 — an independent Medical University of Warsaw review whose Clinical Trials section reports both human studies of the tetrapeptide with their doses, enrollments and comparison groupsretrieved 2026-08-13limited human data