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Reconstitution · Epitalon

Epitalon reconstitution calculator

Pick the vial, the water you added and the dose. Get the exact draw in units for the syringe in your hand.

Step 1 of 4

  1. How many mg are in your vial?
    Vial strengthamount printed on the vial
  2. How many mL of water did you add?
  3. What dose do you want to convert?
  4. Which syringe are you using?
Draw to
10.0units

0.10 mL · 5.00 mg/mL · 50 mcg per unit · 20 doses per vial

10.0 units on a U-100 insulin syringe · 1 mL · lines every 2 units, which is 0.10 milliliters. The draw lands on a syringe line.

Easy to read on this syringe. Draw to the 10-unit line. No rounding is needed.

10 mg ÷ 2 mL = 5.00 mg/mL
50 mcg/unit · 0.5 mg = 0.100 mL = 10.0 units

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Show the math and syringe details

Can this syringe show the result clearly?

Easy to read on this syringe. 10.0 u is exactly the 10-unit line on a U-100 — no rounding error at all.

Want an easier syringe number?

This setup is already easy to read. Changing the water between 0.5 and 3 mL would not put 0.5 mg closer to a line on this U-100 syringe.

Adding more water makes the mixture less concentrated, so the same dose uses more syringe units. It does not change how much peptide is in the vial.

This setup is in the address bar — every input is part of the link.

Educational math, not medical advice — dosing decisions belong to a licensed clinician. The tabs above also plan a schedule week by week and count how many doses one vial holds.

How to use the calculator

  1. STEP 1

    Enter the amount in the vial

    Use the total amount printed on the vial, in mg.

  2. STEP 2

    Enter the water you added

    Use the amount of water added to that vial, in mL.

  3. STEP 3

    Enter the dose you want to convert

    Choose mg or mcg, then match the result to your syringe.

Epitalon is a peptide four amino acids long, made in Leningrad in the 1980s. It copies the amino-acid makeup of an extract of cow pineal gland. It is sold for longevity — longer telomeres, better sleep, slower aging.

The lifespan results people quote were produced by the gland extract, not by this peptide. One monkey experiment ran both side by side and used five hundred times more of the extract. Two human studies of the peptide itself exist. They measured eyesight and a urine marker, and both came out of the institute that made it.

Examples using a 10 mg Epitalon vial

Each row starts with the same 10 mg vial and changes only the amount of water. More water makes the mixture less concentrated, so the same dose uses more syringe units.

◆ How the water amount changes the resultdose used: 0.5 mg
For a 10 milligram vial: bacteriostatic water added, resulting concentration, micrograms per U-100 unit, and the draw for a 0.5 mg dose.
Water addedConcentrationPer U-100 unitDraw for 0.5 mgNearest syringe line
1 mL10.00 mg/mL100.0 mcg5.0 u · 0.050 mL6 u · 20.0% off
2 mL5.00 mg/mL50.0 mcg10.0 u · 0.100 mLexactly 10 u
3 mL3.33 mg/mL33.3 mcg15.0 u · 0.150 mL16 u · 6.7% off

The vial, water and dose shown here are the calculator’s starting example for Epitalon. Replace them with your own values. Every result in the table is calculated from those inputs.

Drawn from once a week at 0.5 mg, a 10 mg vial yields 20 doses — about 20.0 weeks of supply. No cited source says how long the mixed vial keeps, so no storage window is shown.

The studied amount was never injected under the skin

This page opens on the only whole-body amount anybody has published for a person taking this peptide. That amount was held under the tongue. The conversion below is arithmetic, and arithmetic cannot move a finding from one route to another.

4Open the mixing details 4 notes

Two studies, two routes, neither of them this one

One human study held the peptide under the tongue for twenty days. The other injected it into the tissue behind the eye, and that one was an eye treatment judged on eyesight. Neither put anything under the skin.

So the amount this page opens on is real and sourced, and it still does not describe the injection you are mixing for. How much of a peptide reaches the blood depends on the route, and nobody has measured this one.

Every water volume gives a whole number of units

All three listed volumes put the draw on a syringe line. The plunger never has to stop between two lines.

What the water choice changes is how much liquid goes in, and how strong the solution is while it sits in the fridge. It does not change whether the syringe can be read.

Vial strengths are inconsistent between sellers

The page notes that vials sold under this name do not agree on strength. So a dose quoted without its vial size is unusable, and a unit count quoted without both is worse than nothing.

This page can only compute from the strength you enter. If the glass and the invoice disagree, the arithmetic inherits that disagreement and cannot detect it.

The famous survival result was a different substance

The long-running survival reports attached to this peptide tested a bovine gland extract. They did not test the four-residue peptide sold under this name. Different substance, different preparation, different studies.

One monkey experiment gave both side by side and needed five hundred times more of the extract than of the peptide to do it. Two things that far apart inside a single protocol are not one thing.

Both human numbers on this page were read out of a review

Epitalon has human studies, and both of them state a quantity. Neither of the original papers prints one. The figures on this page come from an independent review that read those papers. That puts a link between this page and the source.

4Read the supporting evidence 4 supporting notes

What each original paper actually says

The eye study reports its clinical result and not its dose. It gives neither the patient count nor the ages. The sleep-rhythm study is in Russian, and its abstract states neither dose nor enrollment.

One of the two quantities is not a whole-body dose at all

The smaller of them was given per eye, on consecutive days. It was an eye treatment, judged on eyesight. Per eye is not something this calculator can represent, and no arithmetic turns it into a mass for a person.

The institute that made the peptide ran the trial

Nearly all the work on this molecule comes from two institutes, with the same senior author running through it. The eye trial was run at the institute that created the peptide. So it is not independent of the people who made it.

Most of what was measured was never an outcome in a person

The bulk of this literature sits in dishes and animals. It is melatonin output in cell culture, messenger RNA for an immune signal, mouse thymus cells, and the activity of enzymes. Telomerase is the enzyme people quote.

Units to dose at 5.00 mg/mL

Mix 10 mg into 2 mL and one U-100 unit carries 50.0 mcg. The table shows how much peptide is in each numbered syringe line. These are conversion examples, not suggested doses.

10Open the unit conversion table 10 rows
Numbered U-100 syringe lines converted to volume and mass at 5.00 milligrams per milliliter.
U-100 lineVolumeMass (mcg)Mass (mg)
10 u0.10 mL500 mcg0.5 mg
20 u0.20 mL1,000 mcg1 mg
30 u0.30 mL1,500 mcg1.5 mg
40 u0.40 mL2,000 mcg2 mg
50 u0.50 mL2,500 mcg2.5 mg
60 u0.60 mL3,000 mcg3 mg
70 u0.70 mL3,500 mcg3.5 mg
80 u0.80 mL4,000 mcg4 mg
90 u0.90 mL4,500 mcg4.5 mg
100 u1.00 mL5,000 mcg5 mg

How the math works

  1. Concentration — divide the amount in the vial by the water you added: mg ÷ mL = mg/mL. This tells you how many mg are in each mL.
  2. Volume — the dose divided by that concentration: dose ÷ (mg/mL) = mL.
  3. Units — on a U-100 syringe one unit is 0.01 mL, so mL × 100 = units. A U-40 unit is 0.025 mL, so the wrong barrel is a 2.5× error. That is why the calculator asks which one you have.
  4. Rounding gap — the exact draw against the nearest syringe line: |exact − nearest line| ÷ exact × 100 = % difference.

Medically reviewed by Jennifer Montecillo, MD · non-practicing medical reviewerReviewed 2026-08-13open the primary sources

Useful guides for this calculation

The math is only one part of the question. These guides cover the compound, published doses, side effects, mixing steps and supplies.

1primary source, each with the day we read it — open the documents used on this page