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How-to · reconstitution

How to reconstitute NAD+ 500 mg

Put 3 mL of bacteriostatic water into the 500 mg NAD+ vial. That makes 166.67 mg/mL. A 50 mg dose is then 30.0 units on a U-100 syringe.

That volume is the one that lands the draw on a syringe line, so the reading can be checked by eye. More water makes a weaker mix and a bigger draw. Less water makes a stronger mix and a smaller one. The amount of NAD+ in the glass never changes. Only the number of marks it takes does.

What matters first

Water to add
3 mL
Resulting strength
166.67 mg/mL
Example 50 mg dose
30.0 U-100 units

What is NAD+?

NAD+ is a substance every cell uses to turn food into energy and carry out repair work. It is a coenzyme, not a peptide.

Medically reviewed by Jennifer Montecillo, MD · non-practicing medical reviewerReviewed 2026-08-12open the primary sources

How much bacteriostatic water for a 500 mg NAD+ vial?

◆ Unique to the 500 mg vialU-100 example draws
Water addedConcentrationPer U-100 unitExample: 50 mg =
3 mL166.67 mg/mL1666.67 mcg30.0 units
5 mL100.00 mg/mL1000 mcg50.0 units

Straight arithmetic: concentration = 500 mg ÷ water, and 1 U-100 unit = 0.01 mL. The example column shows the math at 50 mg; it is not a dose recommendation.

Which volume to use is a practical choice rather than a dose. Pick the one that puts your draw somewhere you can actually read it on the syringe. The flags in the last column mark where that fails. “Hard to measure” means the draw is too small to judge by eye. “Won’t fit” means it is bigger than one U-100 syringe holds.

NAD+ 500 mg vial: how much water to add. Each row is what that amount draws on a U-100 syringe. 3 mL makes 166.67 mg/mL, or 1666.67 mcg a unit, and 50 mg is 30 units. 5 mL makes 100.00 mg/mL, or 1000 mcg a unit, and 50 mg is 50 units. The barrel drawn here is filled to 30 units, the draw at 3 mL. The sum is 500 mg divided by the water. One U-100 unit is 0.01 mL.
The 500 mg vial at 3 mL on a U-100 barrel. 50 mg fills to 30.0 units, at 166.67 mg/mL.

How many units is a documented dose from this vial?

The table above uses one worked example. These are the published quantities, converted into marks on a U-100 barrel at each water volume for a 500 mg vial. Where a quantity was never studied by injection, the row says so.

10–50 mg — studied in a trial

10–50 mg once daily for 21 days, into a vein. The three escalating groups (10 mg, then 20 mg, then 50 mg) of a 12-participant phase 1 safety study.

Water addedConcentrationDraw, low to high
3 mL166.67 mg/mL6.0 to 30.0 units
5 mL100.00 mg/mL10.0 to 50.0 units

Human trialNCT06558253 — Phase 1 safety and efficacy study of Coenzyme I (nicotinamide adenine dinucleotide) for injection after single umbilical cord blood transplantation, with 10 mg, 20 mg and 50 mg daily dose groups · retrieved 2026-08-12

100 mg — studied in a trial

100 mg once daily for one week, in a phase 1/2 study in immune thrombocytopenia, into a vein.

Water addedConcentrationDraw
3 mL166.67 mg/mL60.0 units
5 mL100.00 mg/mL100.0 units

Human trialNCT06776510 — A clinical study of intravenous nicotinamide adenine dinucleotide (Coenzyme I for Injection) in the treatment of immune thrombocytopenia, 100 mg/day for one week · retrieved 2026-08-12

500 mg — studied in a trial

500 mg diluted in 500 mL of saline, in a 53-participant comparison against intravenous nicotinamide riboside, into a vein.

Water addedConcentrationDraw
3 mL166.67 mg/mL300.0 unitswon’t fit
5 mL100.00 mg/mL500.0 unitswon’t fit

Human trialNCT06382688 — IV administration of nicotinamide riboside as compared to NAD+: 500 mg of nicotinamide adenine dinucleotide in 500 mL of saline, 53 participants enrolled · retrieved 2026-08-12

Half a gram of powder, and the water is not the whole volume

This is the heaviest vial on the site by a wide margin. At this mass the powder occupies enough room that the liquid you finish with is measurably more than the water you put in.

3Read the full vial record — 3 notes

The table divides by the water, on purpose

Concentration here is vial over water added, because water added is the quantity you measure and control. With a few milligrams of a peptide, the solid contributes nothing you could detect to the final volume.

Half a gram is not in that category. The mix ends up larger than the water, so the true strength sits slightly under the figure in the table. That gap exists on this page and on no other in the family.

Ten draws, in tens of milligrams

The vial divides into ten worked doses, and both water options land exactly on syringe lines.

Everything on this page is an order of magnitude heavier than its neighbors. A decimal point in the wrong place therefore moves the amount much further than it would on a peptide vial. The mass column is the one to read.

Every human amount went into a vein

The registered studies infused this coenzyme under supervision. The consistent finding was discomfort during the infusion itself, bad enough that the drip ran slowly.

Nothing published describes putting it under the skin, which is what the powder sold this way is used for. The arithmetic holds; the route it is being applied to has never been studied.

How do you mix it, step by step?

  1. Wipe both stoppers with an alcohol swab and let them dry.
  2. Draw up the water volume you picked from the table.
  3. Let the water run slowly down the inside wall of the NAD+ vial, rather than jetting it onto the powder.
  4. Swirl gently until it dissolves. Shaking damages peptides.
  5. Store it as the dispensing label directs. What happens after mixing differs by product — some labels direct against refrigerating the mixed solution at all.

What if your water volume is not on the table?

The table shows the offered water volumes. The calculator does the same arithmetic for any volume and any syringe, and shows its working line by line.

Open the NAD+ calculator →

Where to read next about NAD+

The arithmetic on this page is only half the question. These are the pages that carry the other half.

NAD+ — class, half-life and regulatory standing

No approved brand name is listed for NAD+.

Mixing math for any vial, not just NAD+

One calculator for every vial on this site, with NAD+ as one example

Where the NAD+ guides sit in the full list

Vial-specific tables and label-sourced injection guides, including NAD+

NAD+ in the regulatory changelog

A proceeding with no published outcome is entered as exactly that, including NAD+

3primary sources, each with the day we read it — open the documents used on this page