Pick the vial, the water you added and the dose. Get the exact draw in units for the syringe in your hand.
Draw to50.0units0.50 mL
Step 1 of 4
1How many mg are in your vial?
Vial strengthamount printed on the vial
2How many mL of water did you add?
Bacteriostatic water addedhow much water you added
3What dose do you want to convert?
Target doseenter the dose you want to convert
This vial holds 500 mg in total.
4Which syringe are you using?
Syringe typematch the label on your syringe
U-100 insulin syringe · 1 mL · lines every 2 units · 1 unit = 0.01 mL
Draw to
50.0units
0.50 mL · 100.00 mg/mL · 1,000 mcg per unit · 10 doses per vial
50.0 units on a U-100 insulin syringe · 1 mL · lines every 2 units, which is 0.50 milliliters. The draw lands on a syringe line.
Easy to read on this syringe. Draw to the 50-unit line. No rounding is needed.
500 mg ÷ 5 mL = 100.00 mg/mL → 1,000 mcg/unit · 50 mg = 0.500 mL =50.0 units
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Show the math and syringe details+
Can this syringe show the result clearly?
Easy to read on this syringe.50.0 u is exactly the 50-unit line on a U-100 — no rounding error at all.
Want an easier syringe number?
This setup is already easy to read. Changing the water between 0.5 and 3 mL would not put 50 mg closer to a line on this U-100 syringe.
Adding more water makes the mixture less concentrated, so the same dose uses more syringe units. It does not change how much peptide is in the vial.
This setup is in the address bar — every input is part of the link.
Educational math, not medical advice — dosing decisions belong to a licensed clinician. The tabs above also plan a schedule week by week and count how many doses one vial holds.
How to use the calculator
STEP 1
Enter the amount in the vial
Use the total amount printed on the vial, in mg.
STEP 2
Enter the water you added
Use the amount of water added to that vial, in mL.
STEP 3
Enter the dose you want to convert
Choose mg or mcg, then match the result to your syringe.
NAD+ is a substance every cell uses to turn food into energy and carry out repair work. It is a coenzyme, not a peptide. Clinics promote NAD+ IV drips and injections for energy, focus, recovery, hangovers, addiction recovery and healthy aging.
The human studies on NAD+ itself are small and used IV infusions. They have not shown that NAD+ injections improve those goals. The clearest finding so far is discomfort during the drip, including stomach symptoms, a faster heartbeat and chest pressure.
Examples using a 500 mg NAD+ vial
Each row starts with the same 500 mg vial and changes only the amount of water. More water makes the mixture less concentrated, so the same dose uses more syringe units.
◆ How the water amount changes the resultdose used: 50 mg
For a 500 milligram vial: bacteriostatic water added, resulting concentration, micrograms per U-100 unit, and the draw for a 50 mg dose.
Water added
Concentration
Per U-100 unit
Draw for 50 mg
Nearest syringe line
3 mL
166.67 mg/mL
1,666.7 mcg
30.0 u · 0.300 mL
exactly 30 u
5 mL
100.00 mg/mL
1,000.0 mcg
50.0 u · 0.500 mL
exactly 50 u
The vial, water and dose shown here are the calculator’s starting example for NAD+. Replace them with your own values. Every result in the table is calculated from those inputs.
Drawn from once a week at 50 mg, a 500 mg vial yields 10 doses — about 10.0 weeks of supply. No cited source says how long the mixed vial keeps, so no storage window is shown.
Half a gram of powder changes what mixing means
NAD+ is the only thing here measured in hundreds of milligrams. At that mass the powder is bulky enough that the water you add and the liquid you end up with are not the same volume.
3Open the mixing details — 3 notes
The powder takes up room of its own
With a few milligrams of a peptide, the solid adds nothing to the final volume you could measure. Half a gram is different. The solution ends up larger than the water that went in, so the true strength comes out a little weaker than the division suggests.
This page divides by the water, because the water is the number you control and the number you can check. The gap is real, and it belongs to this vial size alone. Nothing else on the site weighs enough for it to matter.
A misplaced decimal travels further here
Everything else on this site draws in micrograms or single milligrams. This page works in tens and hundreds. The same slip of a decimal point therefore moves the dose by a far larger absolute amount.
The page’s own notes flag it. It is the reason to read the mass column on the conversion table rather than trusting a remembered unit count.
Every human amount went into a vein
The registered studies infused it under supervision. The consistent finding in them was discomfort during the infusion itself, bad enough that the drip ran slowly.
Nothing published describes injecting it under the skin, which is how the powder sold under this name gets used. The arithmetic below still works. It is applied to a route nobody has studied.
Three registered trials, and no two of them picked the same amount
NAD+ is the page here whose human numbers come from registered trials and still settle nothing. Three of them chose daily amounts two orders of magnitude apart. Most pages on this site have too little evidence to fix a quantity. This one has evidence that does not converge.
4Read the supporting evidence — 4 supporting notes
Each of the three was answering a different question
One was a small safety study with escalating groups, run after a transplant procedure. One was a short daily course in an immune blood disorder. One was a single infusion set against a precursor molecule.
Most of what a search returns is a different substance
The large majority of human research under this heading studies precursors. They are nicotinamide riboside and nicotinamide mononucleotide, and they are taken by mouth. Those are different molecules on a different route.
Compounded is not approved, and the paperwork says so
Injected NAD+ holds no approval anywhere. Going through a compounding pharmacy is not a shortcut to one. It skips premarket review rather than passing it.
The heaviest vial on the site: who fills it, and what one mark costs
Only one strength is built here, and it is the one confirmed at both a compounding pharmacy and a research vendor. A larger strength has real search demand behind it. No first-party seller has been confirmed for it, so no page is generated.
Units to dose at 100.00 mg/mL
Mix 500 mg into 5 mL and one U-100 unit carries 1,000.0 mcg. The table shows how much peptide is in each numbered syringe line. These are conversion examples, not suggested doses.
10Open the unit conversion table — 10 rows
Numbered U-100 syringe lines converted to volume and mass at 100.00 milligrams per milliliter.
U-100 line
Volume
Mass (mcg)
Mass (mg)
10 u
0.10 mL
10,000 mcg
10 mg
20 u
0.20 mL
20,000 mcg
20 mg
30 u
0.30 mL
30,000 mcg
30 mg
40 u
0.40 mL
40,000 mcg
40 mg
50 u
0.50 mL
50,000 mcg
50 mg
60 u
0.60 mL
60,000 mcg
60 mg
70 u
0.70 mL
70,000 mcg
70 mg
80 u
0.80 mL
80,000 mcg
80 mg
90 u
0.90 mL
90,000 mcg
90 mg
100 u
1.00 mL
100,000 mcg
100 mg
How the math works
Concentration — divide the amount in the vial by the water you added: mg ÷ mL = mg/mL. This tells you how many mg are in each mL.
Volume — the dose divided by that concentration: dose ÷ (mg/mL) = mL.
Units — on a U-100 syringe one unit is 0.01 mL, so mL × 100 = units. A U-40 unit is 0.025 mL, so the wrong barrel is a 2.5× error. That is why the calculator asks which one you have.
Rounding gap — the exact draw against the nearest syringe line: |exact − nearest line| ÷ exact × 100 = % difference.
Medically reviewed by Jennifer Montecillo, MD · non-practicing medical reviewer·Reviewed 2026-08-12·open the primary sources
Useful guides for this calculation
The math is only one part of the question. These guides cover the compound, published doses, side effects, mixing steps and supplies.