Page last checked 2026-08-12

How-to · reconstitution

How to reconstitute Sermorelin 5 mg

Put 2 mL of bacteriostatic water into the 5 mg Sermorelin vial. That makes 2.50 mg/mL. A 500 mcg dose is then 20.0 units on a U-100 syringe.

That volume is the one that lands the draw on a syringe line, so the reading can be checked by eye. More water makes a weaker mix and a bigger draw. Less water makes a stronger mix and a smaller one. The amount of Sermorelin in the glass never changes. Only the number of marks it takes does.

What matters first

Water to add: 2 mL
Resulting strength: 2.50 mg/mL
Example 500 mcg dose: 20.0 U-100 units

What is Sermorelin?

Sermorelin is the first 29 amino acids of the hormone that prompts growth hormone release. It is the shortest piece that still does the job at the pituitary.

Medically reviewed by Jennifer Montecillo, MD · non-practicing medical reviewerReviewed 2026-08-12open the primary sources

How much bacteriostatic water for a 5 mg Sermorelin vial?

◆ Unique to the 5 mg vialU-100 example draws

Water added	Concentration	Per U-100 unit	Example: 500 mcg =
2 mL	2.50 mg/mL	25 mcg	20.0 units
3 mL	1.67 mg/mL	16.67 mcg	30.0 units

Straight arithmetic: concentration = 5 mg ÷ water, and 1 U-100 unit = 0.01 mL. The example column shows the math at 500 mcg; it is not a dose recommendation.

Which volume to use is a practical choice rather than a dose. Pick the one that puts your draw somewhere you can actually read it on the syringe. The flags in the last column mark where that fails. “Hard to measure” means the draw is too small to judge by eye. “Won’t fit” means it is bigger than one U-100 syringe holds.

Sermorelin 5 mg vial: how much water to add. Each row is what that amount draws on a U-100 syringe. 2 mL makes 2.50 mg/mL, or 25 mcg a unit, and 500 mcg is 20 units. 3 mL makes 1.67 mg/mL, or 16.67 mcg a unit, and 500 mcg is 30 units. The barrel drawn here is filled to 20 units, the draw at 2 mL. The sum is 5 mg divided by the water. One U-100 unit is 0.01 mL. — The 5 mg vial at 2 mL on a U-100 barrel. 500 mcg fills to 20.0 units, at 2.50 mg/mL.

How many units is a documented dose from this vial?

The table above uses one worked example. These are the published quantities, converted into marks on a U-100 barrel at each water volume for a 5 mg vial. Where a quantity was never studied by injection, the row says so.

0.5–1 mg — studied in a trial

0.5–1 mg twice daily for 14 days in healthy men of about 68, under the skin. Only the 1 mg arm raised 24-hour growth hormone and IGF-I significantly above the men’s own baseline.

Water added	Concentration	Draw, low to high
2 mL	2.50 mg/mL	20.0 to 40.0 units
3 mL	1.67 mg/mL	30.0 to 60.0 units

Human trialCorpas E, Harman SM, Piñeyro MA, Roberson R, Blackman MR. Growth hormone (GH)-releasing hormone-(1-29) twice daily reverses the decreased GH and insulin-like growth factor-I levels in old men. J Clin Endocrinol Metab 1992;75(2):530-5 · retrieved 2026-08-12

200–300 mcg — reported, not studied

200–300 mcg once daily, as a flat quantity, under the skin. Where every clinical number above was set per kilogram of body weight, and the largest of those trials ran in children rather than in anyone choosing it.

Water added	Concentration	Draw, low to high
2 mL	2.50 mg/mL	8.0 to 12.0 units
3 mL	1.67 mg/mL	12.0 to 18.0 units

indirect evidenceDominikowski A, Rękoś Z, Olejarz M, Szczepanek-Parulska E, Domin R, Ruchała M. The emerging landscape of performance-enhancing peptides modulating GH-IGF1 axis: bridging the gap between clinical evidence and patient self-administration. Front Endocrinol 2026 — tabulates self-reported online dosing patterns beside the clinical doses, stating the reported values must not be read as clinically validated, regulatory-approved or safe · retrieved 2026-08-12

The smallest of four sizes, and the easiest to read

The vial contains ten worked doses, and both water options land exactly on syringe lines. It is the simplest of the four sermorelin vial sizes to measure.

3Read the full vial record — 3 notes

Both volumes read as whole numbers

Each listed volume puts the draw on a graduation, and both sit in the comfortable middle stretch of a U-100 barrel.

That does not survive the rest of the ladder. Three of sermorelin’s four sizes have at least one volume that lands between marks, and one has no clean reading at all.

The vial size is the thing most likely to change

Sermorelin reaches people through compounding pharmacies, which fill it in sizes chosen to suit a course rather than a product line. The strength can differ between one order and the next while the amount stays the same.

A unit count carried over from a previous vial is then wrong, and nothing about the new glass announces it. This page is written for one size only, and the title says which.

Most published amounts scale with weight

The quantities on this page come from trials rather than current labeling. Most of them scale with body weight, so they do not convert into a draw on their own.

The one adult study using a flat amount ran two weeks and measured hormone levels. That is evidence about hormones, not a number to fill a syringe to.

How do you mix it, step by step?

Wipe both stoppers with an alcohol swab and let them dry.
Draw up the water volume you picked from the table.
Let the water run slowly down the inside wall of the Sermorelin vial, rather than jetting it onto the powder.
Swirl gently until it dissolves. Shaking damages peptides.
Store it as the dispensing label directs. What happens after mixing differs by product — some labels direct against refrigerating the mixed solution at all.

What if your water volume is not on the table?

The table shows the offered water volumes. The calculator does the same arithmetic for any volume and any syringe, and shows its working line by line.

Open the Sermorelin calculator →

Questions readers ask

What not to mix with sermorelin?

Nobody has published an interaction list for sermorelin, so this site does not carry one. The approved product left the market years ago, and no current labeling exists for any use.

Mixing two powders in one syringe is a compatibility question, and no published data answers it for this peptide. Building a list from what the molecule does, or from what a similar peptide does, would mean inventing safety information.

How long will 5mg of sermorelin last?

Ten draws at the 500 micrograms in the table, which is the low arm of the one adult trial on this page. That trial gave it twice daily, so ten draws is five days at its rate.

The trial ran for two weeks, but it did not report a storage window for a mixed vial. The result is only a dose count, not a schedule.

How much water do I mix with sermorelin?

With 2 mL or 3 mL of water, 500 micrograms equals 20 units or 30 units. Both land on printed syringe lines.

The vial strength is the part most likely to move. Compounding pharmacies fill sermorelin at sizes chosen for a course, so the next vial may not be 5 mg. A unit count carried over from this page would then be wrong.

How much sermorelin to inject daily?

No label sets a daily amount, because the approved product was withdrawn and no readable labeling survived on an FDA host.

Children with growth failure got 30 micrograms per kilogram once daily. Older men got 0.5 or 1 mg twice daily for two weeks, and only the higher arm moved growth hormone. The figure circulating today, 200 to 300 micrograms once daily, matches neither.

Where to read next about Sermorelin

The arithmetic on this page is only half the question. These are the pages that carry the other half.

Sermorelin research, dosing, and approval status

No regulator has approved sermorelin; the guide separates human studies from reported use.

Check the same draw on a vial that is not sermorelin

Any vial at all, sermorelin among them, with the arithmetic shown rather than hidden.

The dated regulatory page for sermorelin and the rest

Every entry cites its source. It comes from the Federal Register or fda.gov, with sermorelin as context

Every mixing and injection guide, including sermorelin

One page per vial size, because the math differs per vial, with sermorelin as one example

4primary sources, each with the day we read it — open the documents used on this page