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Reconstitution · SS-31

SS-31 reconstitution calculator

Pick the vial, the water you added and the dose. Get the exact draw in units for the syringe in your hand.

Step 1 of 4

  1. How many mg are in your vial?
    Vial strengthamount printed on the vial
  2. How many mL of water did you add?
  3. What dose do you want to convert?
  4. Which syringe are you using?
Draw to
200.0units

2.00 mL · 2.50 mg/mL · 25 mcg per unit · 1 doses per vial

200.0 units on a U-100 insulin syringe · 1 mL · lines every 2 units, which is 2.00 milliliters. The nearest syringe line is 100 units.

Does not fit this syringe. 200.0 units will not fit a U-100 syringe, which holds 100.

5 mg ÷ 2 mL = 2.50 mg/mL
→ 25 mcg/unit · 5 mg = 2.000 mL = 200.0 units

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Show the math and syringe details

Can this syringe show the result clearly?

This draw falls between syringe lines. The exact volume is 200.00 units, but a U-100 syringe has a line every 2 units. Using the nearest line gives 100 units — a 50.0% difference, or 2,500 mcg.

Want an easier syringe number?

  • 0.5 mL of water instead10.00 mg/mL · 100 mcg/unit50.0 uon a syringe line
  • 1 mL of water instead5.00 mg/mL · 50 mcg/unit100.0 uon a syringe line

Adding more water makes the mixture less concentrated, so the same dose uses more syringe units. It does not change how much peptide is in the vial.

This setup is in the address bar — every input is part of the link.

Educational math, not medical advice — dosing decisions belong to a licensed clinician. The tabs above also plan a schedule week by week and count how many doses one vial holds.

How to use the calculator

  1. STEP 1

    Enter the amount in the vial

    Use the total amount printed on the vial, in mg.

  2. STEP 2

    Enter the water you added

    Use the amount of water added to that vial, in mL.

  3. STEP 3

    Enter the dose you want to convert

    Choose mg or mcg, then match the result to your syringe.

SS-31 is a peptide four amino acids long. It gathers inside mitochondria, the parts of a cell that make energy, and sticks to a fat there. It is sold online for energy, mitochondrial health and slower aging.

The same molecule is an approved medicine for a rare inherited condition, at 40 mg a day under the skin. It was tested for the reason people buy it. In 41 older adults with failing mitochondria, it moved energy production about as much as placebo. The vials sold for that use hold 5 mg, an eighth of one day.

Examples using a 5 mg SS-31 vial

Each row starts with the same 5 mg vial and changes only the amount of water. More water makes the mixture less concentrated, so the same dose uses more syringe units.

◆ How the water amount changes the resultdose used: 5 mg
For a 5 milligram vial: bacteriostatic water added, resulting concentration, micrograms per U-100 unit, and the draw for a 5 mg dose.
Water addedConcentrationPer U-100 unitDraw for 5 mgNearest syringe line
1 mL5.00 mg/mL50.0 mcg100.0 u · 1.000 mLexactly 100 u
2 mL2.50 mg/mL25.0 mcg200.0 u · 2.000 mLpast the 100 u barrel

The vial, water and dose shown here are the calculator’s starting example for SS-31. Replace them with your own values. Every result in the table is calculated from those inputs.

Drawn from once a week at 5 mg, a 5 mg vial yields 1 doses — about 1.0 weeks of supply. No cited source says how long the mixed vial keeps, so no storage window is shown.

The approved medicine and the vial on your desk are the same molecule

Almost every compound here is unapproved, and the tool works out what a research vial makes. This one is different. The molecule in this vial is also a licensed medicine, sold under a brand name, with a printed amount behind it. That amount and this vial were never meant to meet, and the arithmetic on this page is where the mismatch becomes visible.

5Read the supporting evidence — 5 supporting notes

One amount, repeated everywhere, and it never changes

Most records here carry a spread. A low figure, a high one, and a range that people move around inside. This one does not.

Why the numbers this page returns look wrong

Enter the published amount against this vial and the barrel runs out. The tool reports that the draw exceeds the syringe, and it is right.

What the opening setting is, and what it is not

The tool has to open on something. On this page it opens on the whole vial.

The gap the tool cannot measure at all

The license covers one rare inherited condition, in patients above a stated weight. It was granted early, on a measurement of leg strength rather than on how anyone lived.

What is in the vial is its own unknown

There is a second gap underneath the first. The licensed product is made to a standard somebody enforces. The research vial is not.

Units to dose at 2.50 mg/mL

Mix 5 mg into 2 mL and one U-100 unit carries 25.0 mcg. The table shows how much peptide is in each numbered syringe line. These are conversion examples, not suggested doses.

10Open the unit conversion table — 10 rows
Numbered U-100 syringe lines converted to volume and mass at 2.50 milligrams per milliliter.
U-100 lineVolumeMass (mcg)Mass (mg)
10 u0.10 mL250 mcg0.25 mg
20 u0.20 mL500 mcg0.5 mg
30 u0.30 mL750 mcg0.75 mg
40 u0.40 mL1,000 mcg1 mg
50 u0.50 mL1,250 mcg1.25 mg
60 u0.60 mL1,500 mcg1.5 mg
70 u0.70 mL1,750 mcg1.75 mg
80 u0.80 mL2,000 mcg2 mg
90 u0.90 mL2,250 mcg2.25 mg
100 u1.00 mL2,500 mcg2.5 mg

How the math works

  1. Concentration — divide the amount in the vial by the water you added: mg ÷ mL = mg/mL. This tells you how many mg are in each mL.
  2. Volume — the dose divided by that concentration: dose ÷ (mg/mL) = mL.
  3. Units — on a U-100 syringe one unit is 0.01 mL, so mL × 100 = units. A U-40 unit is 0.025 mL, so the wrong barrel is a 2.5× error. That is why the calculator asks which one you have.
  4. Rounding gap — the exact draw against the nearest syringe line: |exact − nearest line| ÷ exact × 100 = % difference.

Medically reviewed by Jennifer Montecillo, MD · non-practicing medical reviewerReviewed 2026-08-13open the primary sources

Useful guides for this calculation

The math is only one part of the question. These guides cover the compound, published doses, side effects, mixing steps and supplies.

How SS-31 differs from the rest of its class

No published human dosing schedule was found for SS-31.

Every reaction on the SS-31 page, with its frequency

4 common and 1 serious SS-31 reactions, each carrying its own source.

Reconstituting SS-31 at 5 mg, step by step

The 5 mg SS-31 table itself, not a general rule applied to an unrelated vial size.

Mixing math for any vial, not just SS-31

One calculator for every vial on this site, with SS-31 as one example

Pen needles before the SS-31 arithmetic starts

Which cartons include one and which do not, read off a federal page rather than off an SS-31 page.

What the SS-31 draw needs from bacteriostatic water

What a label allows, stated for bacteriostatic water rather than for SS-31.

1primary source, each with the day we read it — open the documents used on this page