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Reconstitution · SS-31

SS-31 reconstitution calculator

Pick the vial, the water you added and the dose. Get the exact draw in units for the syringe in your hand.

Step 1 of 4

  1. How many mg are in your vial?
    Vial amounttotal mg in the calculation
  2. How many mL of water did you add?
  3. What dose do you want to convert?
  4. Which syringe are you using?
Draw to
200.0units

2.00 mL in the syringe · 1 calculated dose from this amount

200.0 units on a U-100 insulin syringe · 1 mL · lines every 2 units, which is 2.00 milliliters. The nearest syringe line is 100 units.

Does not fit this syringe. 200.0 units will not fit this U-100 syringe, which holds 100 units.

5 mg ÷ 2 mL = 2.50 mg/mL
→ 25 mcg/unit · 5 mg = 2.000 mL = 200.0 units

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Show the math and syringe details

Can this syringe show the result clearly?

This draw falls between syringe lines. The exact volume is 200.00 units, but a U-100 syringe has a line every 2 units. Using the nearest line gives 100 units — a 50.0% difference, or 2,500 mcg.

Want an easier syringe number?

  • 0.5 mL of water instead10.00 mg/mL · 100 mcg/unit50.0 uon a syringe line
  • 1 mL of water instead5.00 mg/mL · 50 mcg/unit100.0 uon a syringe line

Adding more water makes the mixture less concentrated, so the same dose uses more syringe units. It does not change how much peptide is in the vial.

This setup is in the address bar — every input is part of the link.

Educational math, not medical advice — dosing decisions belong to a licensed clinician. The available tabs also show supply calculations and any sourced schedule.

How to use the calculator

  1. 1

    Enter the amount in the vial

    Use the total mg printed on your SS-31 vial.

  2. 2

    Enter the water you added

    Use the amount of water added to that vial, in mL.

  3. 3

    Enter the dose you want to convert

    Choose mg or mcg, then match the result to your syringe.

SS-31 is a peptide four amino acids long. It gathers inside mitochondria, the parts of a cell that make energy, and sticks to a fat there. It is sold online for energy, mitochondrial health and slower aging.

The same molecule is an approved medicine for a rare inherited condition, at 40 mg a day under the skin. It was tested for the reason people buy it. In 41 older adults with failing mitochondria, it moved energy production about as much as placebo. The vials sold for that use hold 5 mg, an eighth of one day.

Examples using 5 mg of SS-31

Each row uses 5 mg and the liquid volume shown. Dividing the amount by that volume gives the calculated concentration. The rows are arithmetic examples, not alternative product preparations.

◆ How liquid volume changes the resultdose used: 5 mg

With 1 mL bacteriostatic water

100.0 units

Concentration
5.00 mg/mL
Each U-100 unit
50.0 mcg
Syringe volume
1.000 mL
Nearest line
Exactly 100 units

With 2 mL bacteriostatic water

200.0 units

Starting example
Concentration
2.50 mg/mL
Each U-100 unit
25.0 mcg
Syringe volume
2.000 mL
Nearest line
Past 100 units

The amount, liquid volume and dose shown here are the calculator’s starting example for SS-31. Replace them with your own values. Every result in the table is calculated from those inputs.

Drawn from once a week at 5 mg, a 5 mg vial yields 1 doses — about 1.0 weeks of supply. No storage limit is assigned to this example. Follow the dispensing label for the specific product.

The Forzinity label does not describe a powder vial

Elamipretide, also called SS-31, is the active ingredient in Forzinity. The approved medicine comes as a prepared solution for Barth syndrome. Its dose instructions do not establish the contents or use of a separate powder vial.

Read the supporting evidence3 supporting notes

The label includes a kidney-related dose reduction

The label gives a usual daily dose and a lower one for some adults with severe kidney problems. The lower dose is for those who are not on dialysis. The dose table states these limits. There is no single amount that applies to everyone.

Adding water cannot increase the amount in a vial

Adding water makes the liquid less concentrated. It does not add more of the drug. If the requested amount exceeds everything in the vial, changing the water or choosing a larger syringe cannot supply the missing mass. A capacity warning identifies a calculation limit.

An example entry is not a prescribed dose

The opening value is an arithmetic example. It does not replace the medicine’s instructions or establish a use for energy, aging or another condition. The math cannot tell you what is in a powder vial, how strong it is or how long it keeps.

Units to dose at 2.50 mg/mL

Mix 5 mg into 2 mL and one U-100 unit carries 25.0 mcg. The table shows how much peptide is in each numbered syringe line. These are conversion examples, not suggested doses.

Open the unit conversion table10 rows
Numbered U-100 syringe lines converted to volume and mass at 2.50 milligrams per milliliter.
U-100 lineVolumeMass (mcg)Mass (mg)
10 u0.10 mL250 mcg0.25 mg
20 u0.20 mL500 mcg0.5 mg
30 u0.30 mL750 mcg0.75 mg
40 u0.40 mL1,000 mcg1 mg
50 u0.50 mL1,250 mcg1.25 mg
60 u0.60 mL1,500 mcg1.5 mg
70 u0.70 mL1,750 mcg1.75 mg
80 u0.80 mL2,000 mcg2 mg
90 u0.90 mL2,250 mcg2.25 mg
100 u1.00 mL2,500 mcg2.5 mg

How the math works

  1. Concentration — divide the amount entered by the liquid volume used in the calculation: mg ÷ mL = mg/mL. This tells you how many mg are in each mL.
  2. Volume — the dose divided by that concentration: dose ÷ (mg/mL) = mL.
  3. Units — on a U-100 syringe one unit is 0.01 mL, so mL × 100 = units. A U-40 unit is 0.025 mL, so the wrong barrel is a 2.5× error. That is why the calculator asks which one you have.
  4. Rounding gap — the exact draw against the nearest syringe line: |exact − nearest line| ÷ exact × 100 = % difference.

Medically reviewed by Kenneth Montecillo, MD2026-09-07open the primary sources

Useful guides for this calculation

The math is only one part of the question. These guides cover the compound, published doses, side effects, mixing steps and supplies.

1primary source, each with the day we read it — open the documents used on this page