Pick the vial, the water you added and the dose. Get the exact draw in units for the syringe in your hand.
Draw to30.0units0.30 mL
Step 1 of 4
1How many mg are in your vial?
Vial strengthamount printed on the vial
2How many mL of water did you add?
Bacteriostatic water addedhow much water you added
3What dose do you want to convert?
Target doseenter the dose you want to convert
This vial holds 10 mg in total.
4Which syringe are you using?
Syringe typematch the label on your syringe
U-100 insulin syringe · 1 mL · lines every 2 units · 1 unit = 0.01 mL
Draw to
30.0units
0.30 mL · 3.33 mg/mL · 33 mcg per unit · 10 doses per vial
30.0 units on a U-100 insulin syringe · 1 mL · lines every 2 units, which is 0.30 milliliters. The draw lands on a syringe line.
Easy to read on this syringe. Draw to the 30-unit line. No rounding is needed.
10 mg ÷ 3 mL = 3.33 mg/mL → 33 mcg/unit · 1 mg = 0.300 mL =30.0 units
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Show the math and syringe details+
Can this syringe show the result clearly?
Easy to read on this syringe.30.0 u is exactly the 30-unit line on a U-100 — no rounding error at all.
Want an easier syringe number?
This setup is already easy to read. Changing the water between 0.5 and 3 mL would not put 1 mg closer to a line on this U-100 syringe.
Adding more water makes the mixture less concentrated, so the same dose uses more syringe units. It does not change how much peptide is in the vial.
This setup is in the address bar — every input is part of the link.
Educational math, not medical advice — dosing decisions belong to a licensed clinician. The tabs above also plan a schedule week by week and count how many doses one vial holds.
How to use the calculator
STEP 1
Enter the amount in the vial
Use the total amount printed on the vial, in mg.
STEP 2
Enter the water you added
Use the amount of water added to that vial, in mL.
STEP 3
Enter the dose you want to convert
Choose mg or mcg, then match the result to your syringe.
TB-500 is a code name for material sold as thymosin beta-4, a protein your body releases where you are injured. The FDA says what is sold under the name is a seven-amino-acid piece of that protein. People buy it for the same injuries as BPC-157 — tendon, muscle and joint — and often buy the two together.
The full protein has been given to people in trials for eye, wound and heart conditions. The short piece sold as TB-500 has not. The FDA states it found no record of any person receiving it, by any route.
Examples using a 10 mg TB-500 vial
Each row starts with the same 10 mg vial and changes only the amount of water. More water makes the mixture less concentrated, so the same dose uses more syringe units.
◆ How the water amount changes the resultdose used: 1 mg
For a 10 milligram vial: bacteriostatic water added, resulting concentration, micrograms per U-100 unit, and the draw for a 1 mg dose.
Water added
Concentration
Per U-100 unit
Draw for 1 mg
Nearest syringe line
2 mL
5.00 mg/mL
50.0 mcg
20.0 u · 0.200 mL
exactly 20 u
3 mL
3.33 mg/mL
33.3 mcg
30.0 u · 0.300 mL
exactly 30 u
The vial, water and dose shown here are the calculator’s starting example for TB-500. Replace them with your own values. Every result in the table is calculated from those inputs.
Drawn from once a week at 1 mg, a 10 mg vial yields 10 doses — about 10.0 weeks of supply. No cited source says how long the mixed vial keeps, so no storage window is shown.
Two different molecules, one name on the vial
The human research people cite for TB-500 ran on a full-length protein. The FDA identifies the material sold under this name as a short fragment of it, and says it has found no human exposure data for that fragment at all.
3Open the mixing details — 3 notes
The trials were not on what is in the vial
The parent protein reached people as eye drops, as gels and as an infusion for a heart study. None of those was a powder vial anybody mixed, and none reached approval.
FDA found no human exposure data for the fragment sold under this name by any route. Evidence often quoted for it belongs to a different molecule.
Milligram draws sit high on the barrel
The example starts at a whole milligram rather than a few hundred micrograms. That produces a longer draw at every listed water volume, so rounding to the nearest syringe line changes very little.
The cost of that comfort is speed. A vial holding a handful of milligrams empties in a handful of doses. Vial life, not readability, is the number to watch on this page.
The mistake: importing the parent protein’s figures
Every injected human amount of the full protein scaled with body weight and went into a vein. Those amounts are small — under a couple of milligrams for an adult, even at the top.
Copying them onto this vial changes the molecule and the route in one move. Either change alone would already break the comparison.
An empty dose list, and a milligram ladder that may never have happened
No published human dose or range from reported practice was found. The calculator starts with a round sample number only to demonstrate the math; it is not guidance.
4Read the supporting evidence — 4 supporting notes
Empty here is a decision, and somebody wrote down the search
A dose row is added when a human quantity survives two questions. Was it this molecule, and was it this route. Every candidate for this compound fails at least one, so the list stays empty.
The one milligram-scale ladder in print is contradicted by its own registry
A published paper reports cohorts of healthy volunteers given milligram-scale amounts into a vein. They were dosed once, then daily for two weeks. Those are the only human quantities near the scale a vial like this gets divided into.
Clumping is the regulator’s stated worry, and clumping is a mixing problem
The agency’s entry for this fragment names two concerns. One is that no human exposure data exists. The other is aggregation — peptide molecules sticking to each other in solution.
No amount means no interval, and the supply figure needs both
Weeks of supply is a division with two inputs. One is how much leaves the vial each time. The other is how often. This page supplies neither.
Units to dose at 3.33 mg/mL
Mix 10 mg into 3 mL and one U-100 unit carries 33.3 mcg. The table shows how much peptide is in each numbered syringe line. These are conversion examples, not suggested doses.
10Open the unit conversion table — 10 rows
Numbered U-100 syringe lines converted to volume and mass at 3.33 milligrams per milliliter.
U-100 line
Volume
Mass (mcg)
Mass (mg)
10 u
0.10 mL
333 mcg
0.333 mg
20 u
0.20 mL
667 mcg
0.667 mg
30 u
0.30 mL
1,000 mcg
1 mg
40 u
0.40 mL
1,333 mcg
1.333 mg
50 u
0.50 mL
1,667 mcg
1.667 mg
60 u
0.60 mL
2,000 mcg
2 mg
70 u
0.70 mL
2,333 mcg
2.333 mg
80 u
0.80 mL
2,667 mcg
2.667 mg
90 u
0.90 mL
3,000 mcg
3 mg
100 u
1.00 mL
3,333 mcg
3.333 mg
How the math works
Concentration — divide the amount in the vial by the water you added: mg ÷ mL = mg/mL. This tells you how many mg are in each mL.
Volume — the dose divided by that concentration: dose ÷ (mg/mL) = mL.
Units — on a U-100 syringe one unit is 0.01 mL, so mL × 100 = units. A U-40 unit is 0.025 mL, so the wrong barrel is a 2.5× error. That is why the calculator asks which one you have.
Rounding gap — the exact draw against the nearest syringe line: |exact − nearest line| ÷ exact × 100 = % difference.
Medically reviewed by Jennifer Montecillo, MD · non-practicing medical reviewer·Reviewed 2026-08-13
Useful guides for this calculation
The math is only one part of the question. These guides cover the compound, published doses, side effects, mixing steps and supplies.
2 mL or 3 mL, across a 5 mg or a 10 mg vial. That gives 2.5 and 1.67 mg/mL for the smaller vial, 5 and 3.33 for the larger.
The example starts at a whole milligram, so all four draws fall between 20 and 60 units. Rounding to the nearest syringe line changes very little at that size. No source establishes that milligram amount as a dose.
What is the recommended dosing protocol for TB 500 peptide?
There is no protocol, and the flattest statement of that comes from the FDA. The agency identifies the substance sold under this name as a seven-residue fragment, LKKTETQ. It states it has found no human exposure data for that fragment by any route.
A check of the whole PubMed record for the name and its sequence, run in August 2026, turned up detection limits rather than doses. The only published dosing of the fragment itself went into two racehorses. They received 10 mg under the skin, in a study built to make a drug test for it.
How to reconstitute 10mg BPC-157 TB 500?
A vial containing two peptides is different from mixing either one alone. The worked example uses 10 mg of each peptide with 2, 3 or 5 mL of water.
At 3 mL each peptide is 3.33 mg/mL, so a single unit carries 33.3 micrograms of both. Whoever filled the vial fixed that ratio. You can set the syringe for one peptide, and the other simply arrives with it.
How long will a 5mg vial of TB 500 last?
Five draws at the 1 mg the tool opens on, and ten from the 10 mg vial. Water moves the reading and never the count.
The 1 mg value is only a worked example, not a recommended dose. No human dose has been established. The only published regimen for the fragment was used in rats, by a different route, so it cannot supply a human quantity.