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Reconstitution · TB-500

TB-500 dosage calculator

Pick the vial, the water you added and the dose. Get the exact draw in units for the syringe in your hand.

Step 1 of 4

  1. How many mg are in your vial?
    Vial amounttotal mg in the calculation
  2. How many mL of water did you add?
  3. What dose do you want to convert?
  4. Which syringe are you using?
Draw to
30.0units

0.30 mL in the syringe · 10 calculated doses from this amount

30.0 units on a U-100 insulin syringe · 1 mL · lines every 2 units, which is 0.30 milliliters. The draw lands on a syringe line.

Easy to read on this syringe. The calculated volume matches the 30-unit line. No rounding is needed.

10 mg ÷ 3 mL = 3.33 mg/mL
→ 33 mcg/unit · 1 mg = 0.300 mL = 30.0 units

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Show the math and syringe details

Can this syringe show the result clearly?

Easy to read on this syringe. 30.0 u is exactly the 30-unit line on a U-100 — no rounding error at all.

Want an easier syringe number?

This setup is already easy to read. Changing the water between 0.5 and 3 mL would not put 1 mg closer to a line on this U-100 syringe.

Adding more water makes the mixture less concentrated, so the same dose uses more syringe units. It does not change how much peptide is in the vial.

This setup is in the address bar — every input is part of the link.

Educational math, not medical advice — dosing decisions belong to a licensed clinician. The available tabs also show supply calculations and any sourced schedule.

How to use the calculator

  1. 1

    Enter the amount in the vial

    Use the total mg printed on your TB-500 vial.

  2. 2

    Enter the water you added

    Use the amount of water added to that vial, in mL.

  3. 3

    Enter the dose you want to convert

    Choose mg or mcg, then match the result to your syringe.

TB-500 is a code name for material sold as thymosin beta-4, a protein your body releases where you are injured. The FDA says what is sold under the name is a seven-amino-acid piece of that protein. People buy it for the same injuries as BPC-157 — tendon, muscle and joint — and often buy the two together.

The full protein has been given to people in trials for eye, wound and heart conditions. The short piece sold as TB-500 has not. The FDA states it found no record of any person receiving it, by any route.

Examples using 10 mg of TB-500

Each row uses 10 mg and the liquid volume shown. Dividing the amount by that volume gives the calculated concentration. The rows are arithmetic examples, not alternative product preparations.

◆ How liquid volume changes the resultdose used: 1 mg

With 2 mL bacteriostatic water

20.0 units

Concentration
5.00 mg/mL
Each U-100 unit
50.0 mcg
Syringe volume
0.200 mL
Nearest line
Exactly 20 units

With 3 mL bacteriostatic water

30.0 units

Starting example
Concentration
3.33 mg/mL
Each U-100 unit
33.3 mcg
Syringe volume
0.300 mL
Nearest line
Exactly 30 units

The amount, liquid volume and dose shown here are the calculator’s starting example for TB-500. Replace them with your own values. Every result in the table is calculated from those inputs.

Drawn from once a week at 1 mg, a 10 mg vial yields 10 doses — about 10.0 weeks of supply. No storage limit is assigned to this example. Follow the dispensing label for the specific product.

TB-500: the compound name matters before the syringe calculation

The TB-500 guide distinguishes the fragment associated with this name from the full-length parent protein. Research on the full-length molecule does not establish a regimen for the fragment. A vial name alone cannot verify its contents.

Open the mixing details3 notes

Keep the molecule and the study together

A study can describe an amount accurately while still being about a different substance from the one a reader has in mind. That distinction is especially important when the parent protein and a shorter fragment appear in the same discussion.

The calculator has no way to identify a peptide or decide which research applies to a product. The linked compound guide explains the identity boundary. Converting an amount to syringe units does not remove it.

Vial strength changes the liquid required

The TB-500 presets include two vial strengths. Holding the amount and water volume steady, the stronger vial produces a more concentrated solution and a smaller calculated draw. A remembered syringe reading cannot carry across that change unchanged.

The displayed examples fit within the selected barrel. Read the target volume and rounding output for the current setup rather than assuming that every different amount will also fit or land on a syringe line.

Count the material separately from the liquid

The theoretical number of draws comes from the vial total divided by the amount entered for each draw. Water changes concentration and draw volume. It does not add more TB-500 or increase that theoretical count.

A small supply count also says nothing about how long a preparation can be stored or which schedule is appropriate. Those are separate questions. The calculation cannot answer them by borrowing a regimen from the parent protein.

TB-500 has no supported human dose row in this reference

This page’s evidence review found no human dose that could be assigned to the TB-500 fragment. Work on full-length thymosin beta-4 does not automatically apply to this shorter peptide. The example entry is there to explain the math.

Read the supporting evidence3 supporting notes

Check the molecule before borrowing a trial amount

Similar names can refer to different peptide sequences. A dose from a study of the full-length peptide cannot become a TB-500 fragment dose just because the two names appear together online. The study material has to match.

An empty dose table is different from an empty calculator

A mass and a liquid volume are enough to work out a concentration. They are not enough to establish a dose. The tool can show the math while the page still states that it has no supported human dose to offer.

There is no evidence-based interval to attach to the example

A supply duration requires both an amount per draw and a frequency. A sample amount does not supply either a validated schedule or a storage window. Those remain separate from the conversion.

Units to dose at 3.33 mg/mL

Mix 10 mg into 3 mL and one U-100 unit carries 33.3 mcg. The table shows how much peptide is in each numbered syringe line. These are conversion examples, not suggested doses.

Open the unit conversion table10 rows
Numbered U-100 syringe lines converted to volume and mass at 3.33 milligrams per milliliter.
U-100 lineVolumeMass (mcg)Mass (mg)
10 u0.10 mL333 mcg0.333 mg
20 u0.20 mL667 mcg0.667 mg
30 u0.30 mL1,000 mcg1 mg
40 u0.40 mL1,333 mcg1.333 mg
50 u0.50 mL1,667 mcg1.667 mg
60 u0.60 mL2,000 mcg2 mg
70 u0.70 mL2,333 mcg2.333 mg
80 u0.80 mL2,667 mcg2.667 mg
90 u0.90 mL3,000 mcg3 mg
100 u1.00 mL3,333 mcg3.333 mg

How the math works

  1. Concentration — divide the amount entered by the liquid volume used in the calculation: mg ÷ mL = mg/mL. This tells you how many mg are in each mL.
  2. Volume — the dose divided by that concentration: dose ÷ (mg/mL) = mL.
  3. Units — on a U-100 syringe one unit is 0.01 mL, so mL × 100 = units. A U-40 unit is 0.025 mL, so the wrong barrel is a 2.5× error. That is why the calculator asks which one you have.
  4. Rounding gap — the exact draw against the nearest syringe line: |exact − nearest line| ÷ exact × 100 = % difference.

Medically reviewed by Kenneth Montecillo, MD2026-09-07

Useful guides for this calculation

The math is only one part of the question. These guides cover the compound, published doses, side effects, mixing steps and supplies.

TB-500 and the limits of animal findings

Animal research or practitioner reports behind the TB-500 safety page.

TB-500 without an approved label: what exists instead

No published half-life figure was found for TB-500.

What a draw carries when TB-500 shares a vial

The ratio was fixed before the vial was bought and one plunger moves both, with TB-500 as context

The stack page TB-500 belongs to

5 traps specific to this pair, written out past TB-500.

Mixing math for any vial, not just TB-500

One calculator for every vial on this site, with TB-500 as one example

What the TB-500 draw needs from the Hospira vial

What a label allows, stated for the Hospira vial rather than for TB-500.

Questions readers ask

How much bacteriostatic water to mix with TB500?

2 mL or 3 mL, across a 5 mg or a 10 mg vial. That gives 2.5 and 1.67 mg/mL for the smaller vial, 5 and 3.33 for the larger.

The example starts at a whole milligram, so all four draws fall between 20 and 60 units. Rounding to the nearest syringe line changes very little at that size. No source establishes that milligram amount as a dose.

What is the recommended dosing protocol for TB 500 peptide?

There is no protocol, and the flattest statement of that comes from the FDA. The agency identifies the substance sold under this name as a seven-residue fragment, LKKTETQ. It states it has found no human exposure data for that fragment by any route.

A check of the whole PubMed record for the name and its sequence, run in August 2026, turned up detection limits rather than doses. The only published dosing of the fragment itself went into two racehorses. They received 10 mg under the skin, in a study built to make a drug test for it.

How to reconstitute 10mg BPC-157 TB 500?

A vial containing two peptides is different from mixing either one alone. The worked example uses 10 mg of each peptide with 2, 3 or 5 mL of water.

At 3 mL each peptide is 3.33 mg/mL, so a single unit carries 33.3 micrograms of both. Whoever filled the vial fixed that ratio. You can set the syringe for one peptide, and the other simply arrives with it.

How long will a 5mg vial of TB 500 last?

Five draws at the 1 mg the tool opens on, and ten from the 10 mg vial. Water moves the reading and never the count.

The 1 mg value is only a worked example, not a recommended dose. No human dose has been established. The only published regimen for the fragment was used in rats, by a different route, so it cannot supply a human quantity.