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Reconstitution · Thymosin alpha-1

Thymosin alpha-1 reconstitution calculator

Pick the vial, the water you added and the dose. Get the exact draw in units for the syringe in your hand.

Step 1 of 4

  1. How many mg are in your vial?
    Vial strengthamount printed on the vial
  2. How many mL of water did you add?
  3. What dose do you want to convert?
  4. Which syringe are you using?
Draw to
32.0units

0.32 mL · 5.00 mg/mL · 50 mcg per unit · 6 doses per vial

32.0 units on a U-100 insulin syringe · 1 mL · lines every 2 units, which is 0.32 milliliters. The draw lands on a syringe line.

Easy to read on this syringe. Draw to the 32-unit line. No rounding is needed.

10 mg ÷ 2 mL = 5.00 mg/mL
50 mcg/unit · 1.6 mg = 0.320 mL = 32.0 units

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Show the math and syringe details

Can this syringe show the result clearly?

Easy to read on this syringe. 32.0 u is exactly the 32-unit line on a U-100 — no rounding error at all.

Want an easier syringe number?

This setup is already easy to read. Changing the water between 0.5 and 3 mL would not put 1.6 mg closer to a line on this U-100 syringe.

Adding more water makes the mixture less concentrated, so the same dose uses more syringe units. It does not change how much peptide is in the vial.

This setup is in the address bar — every input is part of the link.

Educational math, not medical advice — dosing decisions belong to a licensed clinician. The tabs above also plan a schedule week by week and count how many doses one vial holds.

How to use the calculator

  1. STEP 1

    Enter the amount in the vial

    Use the total amount printed on the vial, in mg.

  2. STEP 2

    Enter the water you added

    Use the amount of water added to that vial, in mL.

  3. STEP 3

    Enter the dose you want to convert

    Choose mg or mcg, then match the result to your syringe.

Thymosin alpha-1 is a peptide 28 amino acids long, first pulled out of calf thymus extract in the 1970s. It acts on how T cells mature and signal. It is sold for immune support: long COVID, chronic infection, and general resilience.

It carries a large trial record, and that record is about acutely unwell hospital patients rather than healthy adults. Under the name thymalfasin it is an approved medicine in several countries, mainly for chronic hepatitis B. Its largest trial gave it to 1,106 people with sepsis. By day 28, 23.4% had died on the drug against 24.1% on placebo.

Examples using a 10 mg Thymosin alpha-1 vial

Each row starts with the same 10 mg vial and changes only the amount of water. More water makes the mixture less concentrated, so the same dose uses more syringe units.

◆ How the water amount changes the resultdose used: 1.6 mg
For a 10 milligram vial: bacteriostatic water added, resulting concentration, micrograms per U-100 unit, and the draw for a 1.6 mg dose.
Water addedConcentrationPer U-100 unitDraw for 1.6 mgNearest syringe line
1 mL10.00 mg/mL100.0 mcg16.0 u · 0.160 mLexactly 16 u
2 mL5.00 mg/mL50.0 mcg32.0 u · 0.320 mLexactly 32 u

The vial, water and dose shown here are the calculator’s starting example for Thymosin alpha-1. Replace them with your own values. Every result in the table is calculated from those inputs.

Drawn from once a week at 1.6 mg, a 10 mg vial yields 6 doses — about 6.0 weeks of supply. No cited source says how long the mixed vial keeps, so no storage window is shown.

A settled flat dose, in a vial that holds several of them

Three of the four regimens on this page use the same single quantity. Only the interval changes between them, which is unusual on a research vial — most of the compounds here publish amounts that scale with body weight instead.

3Open the mixing details 3 notes

The trials mixed it differently from the vial you hold

Trials dissolved one dose in a milliliter and injected that amount. The vial sizes shown here contain several times that amount, so one vial covers several trial doses. The studies do not tell you how much water to add to a multi-dose vial.

It also means a figure quoted from the approved labeling elsewhere describes a different container. The milligrams are comparable. The vial is not.

A milligram-scale dose empties a vial fast

Because the amount is milligrams rather than micrograms, a vial holds only a handful of doses. So the question of how long a mixed vial keeps arrives much sooner here than on a compound drawn in micrograms.

No cited source gives a storage window for the mixed vial. The vial-life tool can still count doses and weeks of supply, but it cannot show how long the mixture stays usable.

Approved elsewhere is not approved here

The medicine exists under another name in several countries and has never been approved in the United States. Its trial record is large, and almost all of it ran in hospital in acutely unwell people.

None of those trials describes healthy adults, and the largest of them found no benefit on its main outcome. A real approval somewhere is genuine evidence, and it is not evidence about this use.

The milligrams match the trials; the injection does not

Alone in this group, the amount in the worked example is a quantity people genuinely received. Registered trials used it repeatedly, across several conditions. The problem here is not the number. It is everything arranged around it.

5Read the supporting evidence 5 supporting notes

Same mass, stronger solution, smaller injection

Trials dissolve one dose in a milliliter and inject the lot. The vials sold under this name hold several doses’ worth of powder in a single container.

The number is trial-grade; the compound sits a tier lower

Those two things are not in conflict, and the reason is worth knowing. A figure’s standing comes from where it was measured. A compound’s standing comes from who has reviewed it, and where.

The supply line above assumes a rhythm no trial here used

The intervals on this page run from every twelve hours, through daily, to twice a week. One cancer schedule clusters injections inside a repeating cycle.

The only mixing instruction anyone published says to inject it at once

The trial protocol dissolves each dose immediately before it is given. Nothing in it describes keeping a mixed vial, because in a trial there is never one to keep.

What the arithmetic keeps, and what it will not pick

The division is the one part of this page that does not change with which trial you are reading. Concentration, volume, draw, marks on the barrel: identical across every row on the page.

Units to dose at 5.00 mg/mL

Mix 10 mg into 2 mL and one U-100 unit carries 50.0 mcg. The table shows how much peptide is in each numbered syringe line. These are conversion examples, not suggested doses.

10Open the unit conversion table 10 rows
Numbered U-100 syringe lines converted to volume and mass at 5.00 milligrams per milliliter.
U-100 lineVolumeMass (mcg)Mass (mg)
10 u0.10 mL500 mcg0.5 mg
20 u0.20 mL1,000 mcg1 mg
30 u0.30 mL1,500 mcg1.5 mg
40 u0.40 mL2,000 mcg2 mg
50 u0.50 mL2,500 mcg2.5 mg
60 u0.60 mL3,000 mcg3 mg
70 u0.70 mL3,500 mcg3.5 mg
80 u0.80 mL4,000 mcg4 mg
90 u0.90 mL4,500 mcg4.5 mg
100 u1.00 mL5,000 mcg5 mg

How the math works

  1. Concentration — divide the amount in the vial by the water you added: mg ÷ mL = mg/mL. This tells you how many mg are in each mL.
  2. Volume — the dose divided by that concentration: dose ÷ (mg/mL) = mL.
  3. Units — on a U-100 syringe one unit is 0.01 mL, so mL × 100 = units. A U-40 unit is 0.025 mL, so the wrong barrel is a 2.5× error. That is why the calculator asks which one you have.
  4. Rounding gap — the exact draw against the nearest syringe line: |exact − nearest line| ÷ exact × 100 = % difference.

Medically reviewed by Jennifer Montecillo, MD · non-practicing medical reviewerReviewed 2026-08-12open the primary sources

Useful guides for this calculation

The math is only one part of the question. These guides cover the compound, published doses, side effects, mixing steps and supplies.

Questions readers ask

How much water to add to reconstitute peptides?

Its trial gives a specific preparation: 1.6 mg dissolved in 1 mL and mixed immediately before injection. Few peptide studies publish a mixing volume this clearly.

The research vials do not match that. They hold 5 or 10 mg, so this page’s 1 mL and 2 mL give 10, 5, 5 and 2.5 mg/mL. One trial dose then reads between 16 and 64 units, depending which pairing you mixed.

What to stack with thymosin alpha 1?

One combination has been tested in a registered trial, and it was chemotherapy rather than another peptide. A 488-participant phase 2 in metastatic melanoma gave it alongside dacarbazine and interferon alfa, in arms of 1.6, 3.2 and 6.4 mg.

That is the only trial on this page that varied the quantity at all. No study has paired it with any research peptide. It acts on T-cell function, so it is not a neutral addition for anyone with autoimmune disease or on treatment that suppresses the immune system.

How much thymosin alpha 1 should I take?

The trials converge on one quantity, 1.6 mg under the skin. Every one of them ran in hospital, in acutely unwell people. None describes a healthy adult, and no United States approval exists for any use.

What changes between those trials is the interval rather than the amount. The largest of them, with 1,106 participants, found no survival benefit. This tool turns a quantity you already hold into units, and selects none.

When is the best time to inject thymosin alpha 1?

No trial on this page names a time of day, so nobody can tell you one. What the trials do specify is spacing, and it differs by condition.

Chronic hepatitis B used twice a week, for 26 or 52 weeks. The liver-failure trial ran daily for the first week, then twice a week to week 12. The sepsis trial ran every 12 hours for up to seven days.

4primary sources, each with the day we read it — open the documents used on this page