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Reconstitution · Thymosin alpha-1

Thymosin alpha-1 reconstitution calculator

Pick the vial, the water you added and the dose. Get the exact draw in units for the syringe in your hand.

Step 1 of 4

  1. How many mg are in your vial?
    Vial amounttotal mg in the calculation
  2. How many mL of water did you add?
  3. What dose do you want to convert?
  4. Which syringe are you using?
Draw to
32.0units

0.32 mL in the syringe · 6 calculated doses from this amount

32.0 units on a U-100 insulin syringe · 1 mL · lines every 2 units, which is 0.32 milliliters. The draw lands on a syringe line.

Easy to read on this syringe. The calculated volume matches the 32-unit line. No rounding is needed.

10 mg ÷ 2 mL = 5.00 mg/mL
→ 50 mcg/unit · 1.6 mg = 0.320 mL = 32.0 units

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Show the math and syringe details

Can this syringe show the result clearly?

Easy to read on this syringe. 32.0 u is exactly the 32-unit line on a U-100 — no rounding error at all.

Want an easier syringe number?

This setup is already easy to read. Changing the water between 0.5 and 3 mL would not put 1.6 mg closer to a line on this U-100 syringe.

Adding more water makes the mixture less concentrated, so the same dose uses more syringe units. It does not change how much peptide is in the vial.

This setup is in the address bar — every input is part of the link.

Educational math, not medical advice — dosing decisions belong to a licensed clinician. The available tabs also show supply calculations and any sourced schedule.

How to use the calculator

  1. 1

    Enter the amount in the vial

    Use the total mg printed on your Thymosin alpha-1 vial.

  2. 2

    Enter the water you added

    Use the amount of water added to that vial, in mL.

  3. 3

    Enter the dose you want to convert

    Choose mg or mcg, then match the result to your syringe.

Thymosin alpha-1 is a peptide 28 amino acids long, first pulled out of calf thymus extract in the 1970s. It acts on how T cells mature and signal. It is sold for immune support: long COVID, chronic infection, and general resilience.

It carries a large trial record, and that record is about acutely unwell hospital patients rather than healthy adults. Under the name thymalfasin it is an approved medicine in several countries, mainly for chronic hepatitis B. Its largest trial gave it to 1,106 people with sepsis. By day 28, 23.4% had died on the drug against 24.1% on placebo.

Examples using 10 mg of Thymosin alpha-1

Each row uses 10 mg and the liquid volume shown. Dividing the amount by that volume gives the calculated concentration. The rows are arithmetic examples, not alternative product preparations.

◆ How liquid volume changes the resultdose used: 1.6 mg

With 1 mL bacteriostatic water

16.0 units

Concentration
10.00 mg/mL
Each U-100 unit
100.0 mcg
Syringe volume
0.160 mL
Nearest line
Exactly 16 units

With 2 mL bacteriostatic water

32.0 units

Starting example
Concentration
5.00 mg/mL
Each U-100 unit
50.0 mcg
Syringe volume
0.320 mL
Nearest line
Exactly 32 units

The amount, liquid volume and dose shown here are the calculator’s starting example for Thymosin alpha-1. Replace them with your own values. Every result in the table is calculated from those inputs.

Drawn from once a week at 1.6 mg, a 10 mg vial yields 6 doses — about 6.0 weeks of supply. No storage limit is assigned to this example. Follow the dispensing label for the specific product.

Thymosin alpha-1: the same amount can belong to different regimens

Several Thymosin alpha-1 study entries use the same amount but different intervals and treatment settings. That shared amount is not a universal regimen. The condition, schedule and preparation remain part of each source.

Open the mixing details3 notes

Match the amount with its study schedule

The linked guide separates regimens studied for different conditions. Seeing the same mass in several rows does not mean their frequencies, durations or outcomes can be combined into a new schedule.

The calculator works with an amount per draw. Its vial-life tool also uses an entered frequency to estimate supply. Neither output chooses which study applies to an individual or establishes that a schedule is appropriate outside its original setting.

A study preparation is not every vial preset

The calculator includes two vial-strength presets. They describe totals in a modeled vial, not the amount in each draw. A vial holding several calculated amounts is different from a preparation described for a single administration.

Mixing details belong to the actual preparation. A study amount does not tell the calculator how much water should be used with a different container. The presets illustrate concentrations; they do not replace product-specific instructions.

Supply and usable storage are separate limits

The theoretical draw count comes from vial total divided by the amount per draw. Changing the water volume changes the liquid required each time but leaves that count unchanged. Changing the entered frequency changes the estimated calendar supply.

The record provides no beyond-use period for a mixed vial. A count of draws or weeks therefore cannot establish how long it remains usable. Approval or trial evidence for another preparation does not fill in that missing storage information.

Thymosin alpha-1 studies used different schedules for different conditions

The cited records include hepatitis, sepsis and melanoma studies. Some used the same mass with very different timing; others compared dose levels. There is no single schedule that represents all of these trials.

Read the supporting evidence3 supporting notes

Keep the interval with the condition studied

Daily, twice-weekly and clustered treatment courses produce different exposure patterns. Each schedule belongs to its study population. A matching milligram amount does not make the courses interchangeable.

The study liquid can differ from the calculator example

One protocol specifies a diluent volume for a prepared injection. A vial with another strength produces a different concentration and draw. Trial preparation instructions belong to the studied product, rather than every container with the ingredient’s name.

A trial dose does not predict an individual result

The tool answers a volume question. It cannot choose which condition to treat, predict a benefit or say how long a mixed vial will keep. Those answers do not follow from the amount in a dose.

Units to dose at 5.00 mg/mL

Mix 10 mg into 2 mL and one U-100 unit carries 50.0 mcg. The table shows how much peptide is in each numbered syringe line. These are conversion examples, not suggested doses.

Open the unit conversion table10 rows
Numbered U-100 syringe lines converted to volume and mass at 5.00 milligrams per milliliter.
U-100 lineVolumeMass (mcg)Mass (mg)
10 u0.10 mL500 mcg0.5 mg
20 u0.20 mL1,000 mcg1 mg
30 u0.30 mL1,500 mcg1.5 mg
40 u0.40 mL2,000 mcg2 mg
50 u0.50 mL2,500 mcg2.5 mg
60 u0.60 mL3,000 mcg3 mg
70 u0.70 mL3,500 mcg3.5 mg
80 u0.80 mL4,000 mcg4 mg
90 u0.90 mL4,500 mcg4.5 mg
100 u1.00 mL5,000 mcg5 mg

How the math works

  1. Concentration — divide the amount entered by the liquid volume used in the calculation: mg ÷ mL = mg/mL. This tells you how many mg are in each mL.
  2. Volume — the dose divided by that concentration: dose ÷ (mg/mL) = mL.
  3. Units — on a U-100 syringe one unit is 0.01 mL, so mL × 100 = units. A U-40 unit is 0.025 mL, so the wrong barrel is a 2.5× error. That is why the calculator asks which one you have.
  4. Rounding gap — the exact draw against the nearest syringe line: |exact − nearest line| ÷ exact × 100 = % difference.

Medically reviewed by Kenneth Montecillo, MD2026-09-07open the primary sources

Useful guides for this calculation

The math is only one part of the question. These guides cover the compound, published doses, side effects, mixing steps and supplies.

Thymosin alpha-1 — class, half-life and regulatory standing

No approved brand name is listed for Thymosin alpha-1.

The Thymosin alpha-1 reactions reported most often

Frequency bands exactly as the Thymosin alpha-1 source states them, with grouped figures kept grouped.

Choosing a water volume for a 10 mg Thymosin alpha-1 vial

How the water volume changes concentration, draw size and doses per 10 mg Thymosin alpha-1 vial.

Mixing a 5 mg Thymosin alpha-1 vial, water volume by water volume

What each bacteriostatic-water volume does to a 5 mg Thymosin alpha-1 vial, in mg/mL and units.

Every calculator on this site, Thymosin alpha-1 and the rest

Every intermediate value is printed, for Thymosin alpha-1 or any other vial.

What the Thymosin alpha-1 draw needs from the Zepbound vial draw

What a label allows, stated for the Zepbound vial draw rather than for Thymosin alpha-1.

Questions readers ask

How much water to add to reconstitute peptides?

Its trial gives a specific preparation: 1.6 mg dissolved in 1 mL and mixed immediately before injection. Few peptide studies publish a mixing volume this clearly.

The research vials do not match that. They hold 5 or 10 mg, so this page’s 1 mL and 2 mL give 10, 5, 5 and 2.5 mg/mL. One trial dose then reads between 16 and 64 units, depending which pairing you mixed.

What to stack with thymosin alpha 1?

One combination has been tested in a registered trial, and it was chemotherapy rather than another peptide. A 488-participant phase 2 in metastatic melanoma gave it alongside dacarbazine and interferon alfa, in arms of 1.6, 3.2 and 6.4 mg.

That is the only trial on this page that varied the quantity at all. No study has paired it with any research peptide. It acts on T-cell function, so it is not a neutral addition for anyone with autoimmune disease or on treatment that suppresses the immune system.

How much thymosin alpha 1 should I take?

The trials converge on one quantity, 1.6 mg under the skin. Every one of them ran in hospital, in acutely unwell people. None describes a healthy adult, and no United States approval exists for any use.

What changes between those trials is the interval rather than the amount. The largest of them, with 1,106 participants, found no survival benefit. This tool turns a quantity you already hold into units, and selects none.

When is the best time to inject thymosin alpha 1?

No trial on this page names a time of day, so nobody can tell you one. What the trials do specify is spacing, and it differs by condition.

Chronic hepatitis B used twice a week, for 26 or 52 weeks. The liver-failure trial ran daily for the first week, then twice a week to week 12. The sepsis trial ran every 12 hours for up to seven days.

4primary sources, each with the day we read it — open the documents used on this page